C57BL/6NCya-Cidecem1flox/Cya
Common Name:
Cidec-flox
Product ID:
S-CKO-02492
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cidec-flox
Strain ID
CKOCMP-14311-Cidec-B6N-VA
Gene Name
Product ID
S-CKO-02492
Gene Alias
CIDE-3; Fsp27
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cidecem1flox/Cya mice (Catalog S-CKO-02492) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113089
NCBI RefSeq
NM_001301295
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
CIDEC, also known as cell death-inducing DFF45-like effector C or cell death-inducing DNA fragmentation factor-α-like effector C, is a lipid droplet-associated protein. It plays a crucial role in lipid metabolism, regulating processes such as lipid deposition, secretion, and lipolysis [2,4]. It is involved in maintaining systemic glucose homeostasis and is associated with pathways related to insulin sensitivity [2,3]. Genetic models, like transgenic and knockout mouse models, have been instrumental in studying CIDEC's functions.
CIDEC gene silencing in a type 2 diabetic rat model reversed aortic inflammation and remodeling, suggesting it could be a therapeutic target for diabetic vascular complications [1]. In transgenic mouse models, adipose-specific expression of human CIDEC protected against high-fat diet-induced glucose intolerance and regulated lipid metabolism by interacting with adipose triglyceride lipase's activator, CGI-58 [2]. In a small intestine-specific CIDEC knockout (SI-CIDEC-/-) mouse model, knockout mice had lower body weight, less body fat mass, and reduced liver triglycerides, along with alleviated hepatic steatosis and fatty liver inflammation when fed a high-fat diet. This indicates CIDEC in the small intestine accelerates phosphatidic acid synthesis to promote triglyceride accumulation [5].
In conclusion, CIDEC is a key regulator in lipid metabolism and glucose homeostasis. Studies using gene knockout and transgenic mouse models have revealed its significance in diabetes-related vascular and metabolic diseases, as well as in obesity and hepatic steatosis. These findings highlight CIDEC as a potential therapeutic target for these disease areas.
References:
1. Song, Fang-Qiang, Zhou, Hui-Min, Ma, Wei-Xuan, Zhang, Wei, Ti, Yun. 2022. CIDEC: A Potential Factor in Diabetic Vascular Inflammation. In Journal of vascular research, 59, 114-123. doi:10.1159/000520685. https://pubmed.ncbi.nlm.nih.gov/35124674/
2. Gupta, Abhishek, Balakrishnan, Bijinu, Karki, Shakun, Sharma, Vishva M, Puri, Vishwajeet. 2022. Human CIDEC transgene improves lipid metabolism and protects against high-fat diet-induced glucose intolerance in mice. In The Journal of biological chemistry, 298, 102347. doi:10.1016/j.jbc.2022.102347. https://pubmed.ncbi.nlm.nih.gov/35963433/
3. Balakrishnan, Bijinu, Gupta, Abhishek, Basri, Rabia, Gokce, Noyan, Puri, Vishwajeet. . Endothelial-Specific Expression of CIDEC Improves High-Fat Diet-Induced Vascular and Metabolic Dysfunction. In Diabetes, 72, 19-32. doi:10.2337/db22-0294. https://pubmed.ncbi.nlm.nih.gov/36256836/
4. Li, Yixing, Kang, Huifang, Chu, Yi, Zhao, Shuhong, Zhou, Lei. 2017. Cidec differentially regulates lipid deposition and secretion through two tissue-specific isoforms. In Gene, 641, 265-271. doi:10.1016/j.gene.2017.10.069. https://pubmed.ncbi.nlm.nih.gov/29080839/
5. Huang, Liang, Liao, Qichao, Pan, Tingli, Li, Yixing, Zhou, Lei. 2022. Small Intestine-specific Knockout of CIDEC Improves Obesity and Hepatic Steatosis by Inhibiting Synthesis of Phosphatidic Acid. In International journal of biological sciences, 18, 5740-5752. doi:10.7150/ijbs.74348. https://pubmed.ncbi.nlm.nih.gov/36263170/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen