C57BL/6JCya-Fzd4em1flox/Cya
Common Name:
Fzd4-flox
Product ID:
S-CKO-02510
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fzd4-flox
Strain ID
CKOCMP-14366-Fzd4-B6J-VA
Gene Name
Product ID
S-CKO-02510
Gene Alias
Fz4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fzd4em1flox/Cya mice (Catalog S-CKO-02510) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000058755
NCBI RefSeq
NM_008055
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Fzd4, short for Frizzled-4, is an important receptor for Wnt proteins, which activates several downstream signaling pathways, most notably the Wnt/β -catenin signaling pathway [1,4,5]. This gene is crucial in various biological processes, including the development and maintenance of normal tissues, and its function is associated with stem cell regulation, cell proliferation, and apoptosis [2,4].
In lung cancer, endothelial-specific deletion of FOXF1 in mice decreases Fzd4 expression, which in turn reduces Wnt/β -catenin signaling in tumor-associated endothelial cells (TECs). This leads to decreased pericyte coverage, increased vessel permeability and hypoxia, promoting lung tumor growth and metastasis [1]. In Familial Exudative Vitreoretinopathy (FEVR), whole-gene deletions of Fzd4 have been found in patients, accounting for a certain proportion of cases [3]. Also, in cutaneous squamous cell carcinoma (CSCC), over-expression of Fzd4 in Colo16 cells inhibits cell proliferation and promotes apoptosis, suggesting it may act as a tumor suppressor gene [4].
In conclusion, Fzd4 is essential in maintaining normal physiological functions through its role in Wnt-related signaling pathways. Research using gene-knockout models in mice has revealed its significance in diseases such as lung cancer and FEVR. These findings provide insights into the biological mechanisms underlying these diseases and potential therapeutic targets related to Fzd4.
References:
1. Bian, Fenghua, Goda, Chinmayee, Wang, Guolun, Kalinichenko, Vladimir V, Kalin, Tanya V. 2024. FOXF1 promotes tumor vessel normalization and prevents lung cancer progression through FZD4. In EMBO molecular medicine, 16, 1063-1090. doi:10.1038/s44321-024-00064-8. https://pubmed.ncbi.nlm.nih.gov/38589650/
2. Nabhan, Ahmad N, Webster, Joshua D, Adams, Jarret J, Arron, Joseph R, Dixit, Vishva M. 2023. Targeted alveolar regeneration with Frizzled-specific agonists. In Cell, 186, 2995-3012.e15. doi:10.1016/j.cell.2023.05.022. https://pubmed.ncbi.nlm.nih.gov/37321220/
3. Huang, Li, Lu, Jinglin, Zhang, Linyan, Cao, Liming, Ding, Xiaoyan. 2021. Whole-Gene Deletions of FZD4 Cause Familial Exudative Vitreoretinopathy. In Genes, 12, . doi:10.3390/genes12070980. https://pubmed.ncbi.nlm.nih.gov/34199009/
4. Zhang, Ke, Lv, Qun, Li, Liming, Jiang, Mingjun, Fang, Fang. . Discovering the Role of FZD4 Gene in Human Cutaneous Squamous Cell Carcinoma. In Indian journal of dermatology, 66, 484-489. doi:10.4103/ijd.ijd_1147_20. https://pubmed.ncbi.nlm.nih.gov/35068502/
5. Xiang, Daimin, Gu, Mingye, Liu, Junyu, Fu, Jing, Wang, Hongyang. 2023. m6A RNA methylation-mediated upregulation of HLF promotes intrahepatic cholangiocarcinoma progression by regulating the FZD4/β-catenin signaling pathway. In Cancer letters, 560, 216144. doi:10.1016/j.canlet.2023.216144. https://pubmed.ncbi.nlm.nih.gov/36958694/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen