C57BL/6NCya-G6pc1em1flox/Cya
Common Name:
G6pc1-flox
Product ID:
S-CKO-02519
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
G6pc1-flox
Strain ID
CKOCMP-14377-G6pc1-B6N-VA
Gene Name
Product ID
S-CKO-02519
Gene Alias
G6Pase; G6pc; G6pt; Glc-6-Pase
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-G6pc1em1flox/Cya mice (Catalog S-CKO-02519) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019469
NCBI RefSeq
NM_008061
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
G6pc1, or glucose-6-phosphatase catalytic subunit 1, is crucial for glucose homeostasis as it catalyzes the final rate-limiting step in endogenous glucose production, participating in pathways like gluconeogenesis and glycogenolysis [2,3,6]. This membrane-integrated enzyme, along with accessory transport proteins, hydrolyzes glucose-6-phosphate (G6P) to glucose in the endoplasmic reticulum lumen [1,6,7]. Dysregulation of G6pc1 is associated with diabetes and glycogen storage disease type 1a [3,6,7]. Genetic models, such as mouse models, are valuable for studying its function.
In a humanized mouse model (huR83C) for glycogen storage disease type-Ia, lacking blood glucose control due to a deficient G6PC1 gene, base-editing was able to correct the G6PC1-R83C variant. This restored blood glucose control, improved metabolic abnormalities, and conferred long-term survival, showing that G6pc1 is essential for proper glucose regulation in this disease context [4]. In German Pinschers with glycogen storage disease type Ia (GSD1A), a polyadenine insertion disrupting the G6PC1 gene was identified [5].
In conclusion, G6pc1 is fundamental for maintaining glucose homeostasis through its role in key glucose-related metabolic pathways. Studies using gene-deficient mouse models and other animal models have revealed its critical importance in diseases like glycogen storage disease type 1a. Understanding G6pc1 function provides insights into the mechanisms of glucose-related disorders, potentially guiding the development of therapeutic strategies for such diseases.
References:
1. Hawes, Emily M, Boortz, Kayla A, Oeser, James K, O'Rourke, Margaret L, O'Brien, Richard M. 2023. G6PC1 and G6PC2 influence G6P flux but not HSD11B1 activity. In Journal of molecular endocrinology, 71, . doi:10.1530/JME-23-0070. https://pubmed.ncbi.nlm.nih.gov/37855366/
2. Yamagishi, Genki, Park, Min Kyun, Miyagawa, Shinichi. . Phylogeny of g6pc1 Genes and Their Functional Divergence among Sarcopterygian Vertebrates: Implications for Thermoregulatory Strategies. In Zoological science, 39, 419-430. doi:10.2108/zs210113. https://pubmed.ncbi.nlm.nih.gov/36205363/
3. Tan, Lay Shuen, Lau, Hwee Hui, Abdelalim, Essam M, Tai, E Shyong, Teo, Adrian Kee Keong. 2024. The role of glucose-6-phosphatase activity in glucose homeostasis and its potential for diabetes therapy. In Trends in molecular medicine, 31, 152-164. doi:10.1016/j.molmed.2024.09.005. https://pubmed.ncbi.nlm.nih.gov/39426930/
4. Arnaoutova, Irina, Aratyn-Schaus, Yvonne, Zhang, Lisa, Mansfield, Brian C, Chou, Janice Y. 2024. Base-editing corrects metabolic abnormalities in a humanized mouse model for glycogen storage disease type-Ia. In Nature communications, 15, 9729. doi:10.1038/s41467-024-54108-1. https://pubmed.ncbi.nlm.nih.gov/39523369/
5. Christen, Matthias, Reineking, Wencke, Beineke, Andreas, Baumgärtner, Wolfgang, Leeb, Tosso. 2021. Polyadenine insertion disrupting the G6PC1 gene in German Pinschers with glycogen storage disease type Ia (GSD1A). In Animal genetics, 52, 900-902. doi:10.1111/age.13146. https://pubmed.ncbi.nlm.nih.gov/34610166/
6. Claxton, Derek P, Overway, Emily M, Oeser, James K, O'Brien, Richard M, Mchaourab, Hassane S. 2021. Biophysical and functional properties of purified glucose-6-phosphatase catalytic subunit 1. In The Journal of biological chemistry, 298, 101520. doi:10.1016/j.jbc.2021.101520. https://pubmed.ncbi.nlm.nih.gov/34952005/
7. Xia, Zhanyi, Liu, Chuanyu, Wu, Di, Zhao, Jun, Jiang, Daohua. 2025. Structural insights into glucose-6-phosphate recognition and hydrolysis by human G6PC1. In Proceedings of the National Academy of Sciences of the United States of America, 122, e2418316122. doi:10.1073/pnas.2418316122. https://pubmed.ncbi.nlm.nih.gov/39847333/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen