C57BL/6NCya-Gab2em1flox/Cya
Common Name:
Gab2-flox
Product ID:
S-CKO-02527
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gab2-flox
Strain ID
CKOCMP-14389-Gab2-B6N-VA
Gene Name
Product ID
S-CKO-02527
Gene Alias
D130058I17Rik; p97
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Gab2em1flox/Cya mice (Catalog S-CKO-02527) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000004622
NCBI RefSeq
NM_010248
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Gab2, short for Grb2-associated binder 2, is a scaffolding protein that links plasma membrane receptor signaling to downstream effectors [2]. It is essential for major signal transduction pathways like the PI3-K-AKT and extracellular signal-regulated kinase (ERK) signaling pathways, regulating key cellular processes such as growth, differentiation, migration, and apoptosis [2,6].
In the context of diseases, Gab2 has been implicated in multiple conditions. In deep vein thrombosis (DVT), Gab2 facilitates the assembly of the CBM signalosome in endothelial cells, regulating thromboinflammation. Gene silencing of Gab2 reduced IL-1β-induced responses related to thrombosis in vitro, and Gab2 deficiency suppressed thrombosis in mouse models, suggesting its role in DVT pathogenesis [1]. In cancer, Gab2 promotes the growth and migration of acute myeloid leukemia (AML) cells through the SHP2-Erk-CREB signaling pathway, and Gab2 deficiency prevents Flt3-ITD driven AML in vivo, indicating its carcinogenic role in AML [4,5]. In ovarian cancer, high expression of Gab2 is associated with poor prognosis, and it promotes the proliferation, metastasis, and reduces chemosensitivity of ovarian cancer cells through regulating CrkII [3]. Also, in hepatocellular carcinoma, the tumor suppressor Foxd3 negatively regulates Gab2, and their mutual inhibition controls carcinogenesis [7].
In conclusion, Gab2 plays crucial roles in regulating cellular processes through its involvement in key signaling pathways. Gene knockout or knockdown studies, especially in mouse models, have revealed its significant contributions to diseases such as DVT and various cancers. Understanding Gab2's functions provides potential therapeutic targets for these disease areas.
References:
1. Kondreddy, Vijay, Keshava, Shiva, Das, Kaushik, Rao, L Vijaya Mohan, Pendurthi, Usha R. . The Gab2-MALT1 axis regulates thromboinflammation and deep vein thrombosis. In Blood, 140, 1549-1564. doi:10.1182/blood.2022016424. https://pubmed.ncbi.nlm.nih.gov/35895897/
2. Adams, Sarah J, Aydin, Iraz T, Celebi, Julide T. 2012. GAB2--a scaffolding protein in cancer. In Molecular cancer research : MCR, 10, 1265-70. doi:10.1158/1541-7786.MCR-12-0352. https://pubmed.ncbi.nlm.nih.gov/22871571/
3. Yin, Yi, Zhang, Li, Li, Yong, Zhang, Can, He, Aiqin. 2023. Gab2 plays a carcinogenic role in ovarian cancer by regulating CrkII. In Journal of ovarian research, 16, 79. doi:10.1186/s13048-023-01152-y. https://pubmed.ncbi.nlm.nih.gov/37085900/
4. Gong, Rui, Li, Haoying, Liu, Yaqi, Gu, Haihua, He, Licai. 2022. Gab2 promotes acute myeloid leukemia growth and migration through the SHP2-Erk-CREB signaling pathway. In Journal of leukocyte biology, 112, 669-677. doi:10.1002/JLB.2A0421-221R. https://pubmed.ncbi.nlm.nih.gov/35322464/
5. Spohr, Corinna, Poggio, Teresa, Andrieux, Geoffroy, Illert, Anna L, Brummer, Tilman. 2021. Gab2 deficiency prevents Flt3-ITD driven acute myeloid leukemia in vivo. In Leukemia, 36, 970-982. doi:10.1038/s41375-021-01490-0. https://pubmed.ncbi.nlm.nih.gov/34903841/
6. Ding, Chen-Bo, Yu, Wei-Na, Feng, Ji-Hong, Luo, Jun-Min. 2015. Structure and function of Gab2 and its role in cancer (Review). In Molecular medicine reports, 12, 4007-4014. doi:10.3892/mmr.2015.3951. https://pubmed.ncbi.nlm.nih.gov/26095858/
7. Liu, Ruimin, Sun, Yan, Chen, Shuai, Hong, Yun, Lu, Zhongxian. 2022. FOXD3 and GAB2 as a pair of rivals antagonistically control hepatocellular carcinogenesis. In The FEBS journal, 289, 4536-4548. doi:10.1111/febs.16403. https://pubmed.ncbi.nlm.nih.gov/35178865/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen