C57BL/6JCya-Gad1em1flox/Cya
Common Name:
Gad1-flox
Product ID:
S-CKO-02547
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gad1-flox
Strain ID
CKOCMP-14415-Gad1-B6J-VA
Gene Name
Product ID
S-CKO-02547
Gene Alias
EP10; Gad-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gad1em1flox/Cya mice (Catalog S-CKO-02547) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094934
NCBI RefSeq
NM_008077
Target Region
Exon 6
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Gad1, also known as glutamate decarboxylase 1, is an enzyme that catalyzes the production of γ -aminobutyric acid (GABA) from L -glutamic acid [5]. GABA is a prominent neurotransmitter, and thus Gad1 is crucial for neurotransmission. The gene is also associated with pathways like RAS/MAPK, Wnt/β -catenin, and GABAB receptor -mediated signaling, which are involved in various biological processes [1,2,3,6].
In glioma, overexpression of Gad1 inhibited cell proliferation, invasion, migration, promoted apoptosis, and enhanced sensitivity to cuproptosis by inhibiting the RAS/MAPK pathway [1]. In oral squamous cell carcinoma (OSCC), knockdown of Gad1 expression significantly inhibited cell proliferation, migration, and invasion by reducing GABA -mediated GABAB receptors [3]. In osteosarcoma, a Gad1 inhibitor suppressed tumor growth through the Wnt/β -catenin signaling pathway [6]. In castration -resistant prostate cancer, Gad1 promoted cancer progression and drug resistance [7]. In Gad1 knock -out rats, there were abundant spike -wave discharges in EEG, indicating a role of Gad1 -mediated GABA in suppressing epileptic brain activities [4].
In conclusion, Gad1 plays diverse roles in different biological processes and diseases. Through gene knockout models like in rats, it has been revealed that Gad1 is essential in maintaining normal neural functions, such as suppressing epileptic activities. In cancer, it can either act as a tumor suppressor as in glioma or promote tumor progression as seen in OSCC, osteosarcoma, and castration -resistant prostate cancer, highlighting its complex and context -dependent functions.
References:
1. Gao, Zhiqiang, Yang, Jing. . GAD1 ameliorates glioma progression through regulating cuproptosis via RAS/MAPK pathway. In Journal of biochemical and molecular toxicology, 38, e23848. doi:10.1002/jbt.23848. https://pubmed.ncbi.nlm.nih.gov/39264832/
2. Huang, De, Wang, Yan, Thompson, J Will, Li, Qi-Jing, Wang, Xiao-Fan. 2022. Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. In Nature cell biology, 24, 230-241. doi:10.1038/s41556-021-00820-9. https://pubmed.ncbi.nlm.nih.gov/35145222/
3. Dou, Zhichao, Li, Mao, Shen, Zeliang, Liang, Xinhua, Tang, Yaling. 2023. GAD1-mediated GABA elicits aggressive characteristics of human oral cancer cells. In Biochemical and biophysical research communications, 681, 80-89. doi:10.1016/j.bbrc.2023.09.041. https://pubmed.ncbi.nlm.nih.gov/37774573/
4. Liu, Dongyu, Fujihara, Kazuyuki, Yanagawa, Yuchio, Mushiake, Hajime, Ohshiro, Tomokazu. 2023. Gad1 knock-out rats exhibit abundant spike-wave discharges in EEG, exacerbated with valproate treatment. In Frontiers in neurology, 14, 1243301. doi:10.3389/fneur.2023.1243301. https://pubmed.ncbi.nlm.nih.gov/37830095/
5. Soejima, Shiho, Kondo, Kazuya, Tsuboi, Mitsuhiro, Takizawa, Hiromitsu, Tangoku, Akira. 2021. GAD1 expression and its methylation as indicators of malignant behavior in thymic epithelial tumors. In Oncology letters, 21, 483. doi:10.3892/ol.2021.12744. https://pubmed.ncbi.nlm.nih.gov/33968199/
6. Jian, Changchun, Wang, Ben, Mou, Hai, Huang, Qiu, Ou, Yunsheng. 2024. A GAD1 inhibitor suppresses osteosarcoma growth through the Wnt/β-catenin signaling pathway. In Heliyon, 10, e31444. doi:10.1016/j.heliyon.2024.e31444. https://pubmed.ncbi.nlm.nih.gov/38803976/
7. Wan, Lilin, Liu, Yifan, Liu, Ruiji, Mao, Weipu. 2023. GAD1 contributes to the progression and drug resistance in castration resistant prostate cancer. In Cancer cell international, 23, 255. doi:10.1186/s12935-023-03093-4. https://pubmed.ncbi.nlm.nih.gov/37904122/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen