Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Gad1em1flox/Cya
Common Name:
Gad1-flox
Product ID:
S-CKO-02547
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Gad1-flox
Strain ID
CKOCMP-14415-Gad1-B6J-VA
Gene Name
Gad1
Product ID
S-CKO-02547
Gene Alias
EP10; Gad-1
Background
C57BL/6JCya
NCBI ID
14415
Modification
Conditional knockout
Chromosome
2
Phenotype
MGI:95632
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gad1em1flox/Cya mice (Catalog S-CKO-02547) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094934
NCBI RefSeq
NM_008077
Target Region
Exon 6
Size of Effective Region
~0.9 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Gad1, also known as glutamate decarboxylase 1, is an enzyme that catalyzes the production of γ -aminobutyric acid (GABA) from L -glutamic acid [5]. GABA is a prominent neurotransmitter, and thus Gad1 is crucial for neurotransmission. The gene is also associated with pathways like RAS/MAPK, Wnt/β -catenin, and GABAB receptor -mediated signaling, which are involved in various biological processes [1,2,3,6].

In glioma, overexpression of Gad1 inhibited cell proliferation, invasion, migration, promoted apoptosis, and enhanced sensitivity to cuproptosis by inhibiting the RAS/MAPK pathway [1]. In oral squamous cell carcinoma (OSCC), knockdown of Gad1 expression significantly inhibited cell proliferation, migration, and invasion by reducing GABA -mediated GABAB receptors [3]. In osteosarcoma, a Gad1 inhibitor suppressed tumor growth through the Wnt/β -catenin signaling pathway [6]. In castration -resistant prostate cancer, Gad1 promoted cancer progression and drug resistance [7]. In Gad1 knock -out rats, there were abundant spike -wave discharges in EEG, indicating a role of Gad1 -mediated GABA in suppressing epileptic brain activities [4].

In conclusion, Gad1 plays diverse roles in different biological processes and diseases. Through gene knockout models like in rats, it has been revealed that Gad1 is essential in maintaining normal neural functions, such as suppressing epileptic activities. In cancer, it can either act as a tumor suppressor as in glioma or promote tumor progression as seen in OSCC, osteosarcoma, and castration -resistant prostate cancer, highlighting its complex and context -dependent functions.

References:
1. Gao, Zhiqiang, Yang, Jing. . GAD1 ameliorates glioma progression through regulating cuproptosis via RAS/MAPK pathway. In Journal of biochemical and molecular toxicology, 38, e23848. doi:10.1002/jbt.23848. https://pubmed.ncbi.nlm.nih.gov/39264832/
2. Huang, De, Wang, Yan, Thompson, J Will, Li, Qi-Jing, Wang, Xiao-Fan. 2022. Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. In Nature cell biology, 24, 230-241. doi:10.1038/s41556-021-00820-9. https://pubmed.ncbi.nlm.nih.gov/35145222/
3. Dou, Zhichao, Li, Mao, Shen, Zeliang, Liang, Xinhua, Tang, Yaling. 2023. GAD1-mediated GABA elicits aggressive characteristics of human oral cancer cells. In Biochemical and biophysical research communications, 681, 80-89. doi:10.1016/j.bbrc.2023.09.041. https://pubmed.ncbi.nlm.nih.gov/37774573/
4. Liu, Dongyu, Fujihara, Kazuyuki, Yanagawa, Yuchio, Mushiake, Hajime, Ohshiro, Tomokazu. 2023. Gad1 knock-out rats exhibit abundant spike-wave discharges in EEG, exacerbated with valproate treatment. In Frontiers in neurology, 14, 1243301. doi:10.3389/fneur.2023.1243301. https://pubmed.ncbi.nlm.nih.gov/37830095/
5. Soejima, Shiho, Kondo, Kazuya, Tsuboi, Mitsuhiro, Takizawa, Hiromitsu, Tangoku, Akira. 2021. GAD1 expression and its methylation as indicators of malignant behavior in thymic epithelial tumors. In Oncology letters, 21, 483. doi:10.3892/ol.2021.12744. https://pubmed.ncbi.nlm.nih.gov/33968199/
6. Jian, Changchun, Wang, Ben, Mou, Hai, Huang, Qiu, Ou, Yunsheng. 2024. A GAD1 inhibitor suppresses osteosarcoma growth through the Wnt/β-catenin signaling pathway. In Heliyon, 10, e31444. doi:10.1016/j.heliyon.2024.e31444. https://pubmed.ncbi.nlm.nih.gov/38803976/
7. Wan, Lilin, Liu, Yifan, Liu, Ruiji, Mao, Weipu. 2023. GAD1 contributes to the progression and drug resistance in castration resistant prostate cancer. In Cancer cell international, 23, 255. doi:10.1186/s12935-023-03093-4. https://pubmed.ncbi.nlm.nih.gov/37904122/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest