C57BL/6JCya-Gas1em1flox/Cya
Common Name:
Gas1-flox
Product ID:
S-CKO-02564
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gas1-flox
Strain ID
CKOCMP-14451-Gas1-B6J-VA
Gene Name
Product ID
S-CKO-02564
Gene Alias
Gas-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gas1em1flox/Cya mice (Catalog S-CKO-02564) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000065086
NCBI RefSeq
NM_008086
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Gas1, short for Growth Arrest Specific 1, is a gene with diverse functions. It is a member of the alpha receptors (GFRα) for the GDNF ligands (GFL) family. Gas1 is involved in multiple signaling pathways, such as Wnt, Hedgehog (Hh), and NOTCH, and plays a crucial role in various biological processes including embryonic development, cell growth regulation, and apoptosis [2,4,6]. Genetic models, like gene knockout (KO) mouse models, have been instrumental in studying its functions.
In mice with Gas1 loss-of-function mutation, there are variations in tooth number, morphology, and size within the molar dentition. Gas1 in cranial neural crest cells regulates tooth number by restricting Wnt and downstream FGF signaling in odontogenic epithelium through facilitating Shh signal transduction [3]. In the context of colorectal cancer, Bifidobacterium adolescentis can suppress tumorigenesis by regulating Gas1 via the Wnt signaling pathway, inducing a new subset of CD143+ cancer-associated fibroblasts [1]. Also, in gastric adenocarcinoma, loss of ATOH1 activates the AKT/mTOR signaling pathway through down-regulating Gas1 [5].
In conclusion, Gas1 is a pleiotropic regulator involved in embryonic development, especially in tooth patterning. In disease conditions, it plays a role in cancers such as colorectal and gastric adenocarcinoma. Studies using KO mouse models have significantly contributed to understanding Gas1's functions in these biological processes and disease areas.
References:
1. Chen, Shujie, Fan, Lina, Lin, Yifeng, Si, Jianmin, Wang, Liangjing. 2023. Bifidobacterium adolescentis orchestrates CD143+ cancer-associated fibroblasts to suppress colorectal tumorigenesis by Wnt signaling-regulated GAS1. In Cancer communications (London, England), 43, 1027-1047. doi:10.1002/cac2.12469. https://pubmed.ncbi.nlm.nih.gov/37533188/
2. Segovia, José, Zarco, Natanael. . Gas1 is a pleiotropic regulator of cellular functions: from embryonic development to molecular actions in cancer gene therapy. In Mini reviews in medicinal chemistry, 14, 1139-47. doi:. https://pubmed.ncbi.nlm.nih.gov/25429664/
3. Seppala, M, Thivichon-Prince, B, Xavier, G M, Viriot, L, Cobourne, M T. 2021. Gas1 Regulates Patterning of the Murine and Human Dentitions through Sonic Hedgehog. In Journal of dental research, 101, 473-482. doi:10.1177/00220345211049403. https://pubmed.ncbi.nlm.nih.gov/34796774/
4. Marczenke, Maike, Sunaga-Franze, Daniele Yumi, Popp, Oliver, Allen, Benjamin L, Willnow, Thomas E. 2021. GAS1 is required for NOTCH-dependent facilitation of SHH signaling in the ventral forebrain neuroepithelium. In Development (Cambridge, England), 148, . doi:10.1242/dev.200080. https://pubmed.ncbi.nlm.nih.gov/34698766/
5. Zhong, Qing, Wang, Hua-Gen, Yang, Ji-Hong, Chen, Qi-Yue, Huang, Chang-Ming. 2023. Loss of ATOH1 in Pit Cell Drives Stemness and Progression of Gastric Adenocarcinoma by Activating AKT/mTOR Signaling through GAS1. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2301977. doi:10.1002/advs.202301977. https://pubmed.ncbi.nlm.nih.gov/37824217/
6. Martinelli, David C, Fan, Chen-Ming. 2007. The role of Gas1 in embryonic development and its implications for human disease. In Cell cycle (Georgetown, Tex.), 6, 2650-5. doi:. https://pubmed.ncbi.nlm.nih.gov/17726382/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen