C57BL/6JCya-Gch1em1flox/Cya
Common Name:
Gch1-flox
Product ID:
S-CKO-02588
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gch1-flox
Strain ID
CKOCMP-14528-Gch1-B6J-VA
Gene Name
Product ID
S-CKO-02588
Gene Alias
GTP-CH; GTP-CH-I; GTPCH; Gch
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gch1em1flox/Cya mice (Catalog S-CKO-02588) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000089959
NCBI RefSeq
NM_008102
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Gch1, which codes for GTP cyclohydrolase 1, is a gene with significant biological importance. It regulates the formation of Tetrahydrobiopterin (BH4), a crucial cofactor for the synthesis of essential neurotransmitters like dopamine, serotonin, and nitric oxide synthases. The BH4 synthesis pathway in which Gch1 is involved has a wide-ranging impact on various biological processes, including blood pressure regulation, vasodilatory functions, and oxidative stress [1].
Gch1 deficiency is associated with multiple neuropsychiatric diseases. In humans, certain single-nucleotide polymorphisms of Gch1 are responsible for pain in sickle cell disease, and its deficiency can lead to dopa-responsive dystonia, hyperalaninemia, Parkinson's disease, and depression [1]. In hph-1 mice (a genetic mouse model of DOPA-responsive dystonia with Gch1 mutations), reduced pain sensitivity was observed following peripheral inflammation or capsaicin sensitization, suggesting that Gch1 variants can regulate pain sensitivity, especially after pain sensitization [4]. In amyotrophic lateral sclerosis (ALS), lipid peroxidation of ferroptosis in hSOD1G93A cells and mice was related to inactivation of Gch1. SPY1, a "cyclin-like" protein, was identified as a novel ferroptosis suppressor via alleviating lipid peroxidation produced by dysregulated Gch1/BH4 axis [3]. In glioblastoma, circLRFN5 binds to PRRX2 protein and promotes its degradation, which in turn transcriptionally upregulates Gch1 expression, and Gch1 is a ferroptosis suppressor via generating the antioxidant BH4 [2].
In conclusion, Gch1 is essential for regulating neurotransmitter synthesis and antioxidant-related processes. Model-based research, especially using mouse models with Gch1 mutations, has revealed its role in multiple disease areas, such as neurodegenerative diseases, pain-related conditions, and cancer-associated ferroptosis. Understanding Gch1's functions provides potential therapeutic targets for treating these diseases.
References:
1. Gupta, Parul, Kumar, Ravindra. 2023. GTP cyclohydroxylase1 (GCH1): Role in neurodegenerative diseases. In Gene, 888, 147749. doi:10.1016/j.gene.2023.147749. https://pubmed.ncbi.nlm.nih.gov/37652170/
2. Jiang, Yang, Zhao, Junshuang, Li, Rongqing, Gao, Liang, Cui, Daming. 2022. CircLRFN5 inhibits the progression of glioblastoma via PRRX2/GCH1 mediated ferroptosis. In Journal of experimental & clinical cancer research : CR, 41, 307. doi:10.1186/s13046-022-02518-8. https://pubmed.ncbi.nlm.nih.gov/36266731/
3. Wang, Di, Liang, Weiwei, Huo, Di, Zhang, Dongmei, Feng, Honglin. 2022. SPY1 inhibits neuronal ferroptosis in amyotrophic lateral sclerosis by reducing lipid peroxidation through regulation of GCH1 and TFR1. In Cell death and differentiation, 30, 369-382. doi:10.1038/s41418-022-01089-7. https://pubmed.ncbi.nlm.nih.gov/36443440/
4. Nasser, Arafat, Møller, Lisbeth Birk. 2014. GCH1 variants, tetrahydrobiopterin and their effects on pain sensitivity. In Scandinavian journal of pain, 5, 121-128. doi:10.1016/j.sjpain.2013.12.001. https://pubmed.ncbi.nlm.nih.gov/29913682/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen