C57BL/6JCya-Gdf9em1flox/Cya
Common Name:
Gdf9-flox
Product ID:
S-CKO-02604
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Gdf9-flox
Strain ID
CKOCMP-14566-Gdf9-B6J-VA
Gene Name
Product ID
S-CKO-02604
Gene Alias
Gdf-9
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gdf9em1flox/Cya mice (Catalog S-CKO-02604) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018382
NCBI RefSeq
NM_008110
Target Region
Exon 2
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Growth Differentiation Factor 9 (GDF9) is a member of the transforming growth factor-β (TGF-β) superfamily. It is an oocyte-secreted factor that plays a pivotal role in ovarian function in female reproduction. GDF9 is essential for ovarian follicular growth, regulating processes such as cellular proliferation/differentiation, follicular survival/atresia, and oocyte maturation through paracrine signalling [1,3,4,5].
A Gdf9Q308X/S415T mouse model was generated based on a variant found in an infertile patient. This model recapitulated phenotypes seen in the probands, including abnormal estrogen secretion and defective follicle enlargement. Further experiments in this mouse model showed an earlier expression of STAR in small antral follicles and decreased proliferative capacity in large antral follicles. RNA sequencing of granulosa cells also revealed transcriptomic profiles related to defective follicle enlargement in the Gdf9Q308X/S415T group [2].
In conclusion, GDF9 is crucial for ovarian follicular development and oocyte-related processes. The Gdf9Q308X/S415T mouse model has been instrumental in revealing its role in antral follicle development and associated phenotypes in female subfertility, highlighting the essential role of oocyte-derived GDF9 in ovarian response [2].
References：
1. Liu, Meng-Na, Zhang, Kun, Xu, Tian-Min. 2019. The role of BMP15 and GDF9 in the pathogenesis of primary ovarian insufficiency. In Human fertility (Cambridge, England), 24, 325-332. doi:10.1080/14647273.2019.1672107. https://pubmed.ncbi.nlm.nih.gov/31607184/
2. Duan, Yuwei, Cai, Bing, Guo, Jing, Zhou, Canquan, Xu, Yanwen. 2024. GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement. In Cell communication and signaling : CCS, 22, 235. doi:10.1186/s12964-024-01616-8. https://pubmed.ncbi.nlm.nih.gov/38643161/
3. Belli, Martina, Shimasaki, Shunichi. . Molecular Aspects and Clinical Relevance of GDF9 and BMP15 in Ovarian Function. In Vitamins and hormones, 107, 317-348. doi:10.1016/bs.vh.2017.12.003. https://pubmed.ncbi.nlm.nih.gov/29544636/
4. Juengel, J L, Bodensteiner, K J, Heath, D A, Sawyer, H R, McNatty, K P. . Physiology of GDF9 and BMP15 signalling molecules. In Animal reproduction science, 82-83, 447-60. doi:. https://pubmed.ncbi.nlm.nih.gov/15271472/
5. Paulini, Fernanda, Melo, Eduardo O. 2010. The role of oocyte-secreted factors GDF9 and BMP15 in follicular development and oogenesis. In Reproduction in domestic animals = Zuchthygiene, 46, 354-61. doi:10.1111/j.1439-0531.2010.01739.x. https://pubmed.ncbi.nlm.nih.gov/21198974/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen