C57BL/6JCya-Gdf9em1flox/Cya
Common Name
Gdf9-flox
Product ID
S-CKO-02604
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-14566-Gdf9-B6J-VA
When using this mouse strain in a publication, please cite “Gdf9-flox Mouse (Catalog S-CKO-02604) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gdf9-flox
Strain ID
CKOCMP-14566-Gdf9-B6J-VA
Gene Name
Product ID
S-CKO-02604
Gene Alias
Gdf-9
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000018382
NCBI RefSeq
NM_008110
Target Region
Exon 2
Size of Effective Region
~3.2 kb
Overview of Gene Research
Growth Differentiation Factor 9 (GDF9) is a member of the transforming growth factor-β (TGF-β) superfamily. It is an oocyte-secreted factor that plays a pivotal role in ovarian function in female reproduction. GDF9 is essential for ovarian follicular growth, regulating processes such as cellular proliferation/differentiation, follicular survival/atresia, and oocyte maturation through paracrine signalling [1,3,4,5].
A Gdf9Q308X/S415T mouse model was generated based on a variant found in an infertile patient. This model recapitulated phenotypes seen in the probands, including abnormal estrogen secretion and defective follicle enlargement. Further experiments in this mouse model showed an earlier expression of STAR in small antral follicles and decreased proliferative capacity in large antral follicles. RNA sequencing of granulosa cells also revealed transcriptomic profiles related to defective follicle enlargement in the Gdf9Q308X/S415T group [2].
In conclusion, GDF9 is crucial for ovarian follicular development and oocyte-related processes. The Gdf9Q308X/S415T mouse model has been instrumental in revealing its role in antral follicle development and associated phenotypes in female subfertility, highlighting the essential role of oocyte-derived GDF9 in ovarian response [2].
References：
1. Liu, Meng-Na, Zhang, Kun, Xu, Tian-Min. 2019. The role of BMP15 and GDF9 in the pathogenesis of primary ovarian insufficiency. In Human fertility (Cambridge, England), 24, 325-332. doi:10.1080/14647273.2019.1672107. https://pubmed.ncbi.nlm.nih.gov/31607184/
2. Duan, Yuwei, Cai, Bing, Guo, Jing, Zhou, Canquan, Xu, Yanwen. 2024. GDF9His209GlnfsTer6/S428T and GDF9Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement. In Cell communication and signaling : CCS, 22, 235. doi:10.1186/s12964-024-01616-8. https://pubmed.ncbi.nlm.nih.gov/38643161/
3. Belli, Martina, Shimasaki, Shunichi. . Molecular Aspects and Clinical Relevance of GDF9 and BMP15 in Ovarian Function. In Vitamins and hormones, 107, 317-348. doi:10.1016/bs.vh.2017.12.003. https://pubmed.ncbi.nlm.nih.gov/29544636/
4. Juengel, J L, Bodensteiner, K J, Heath, D A, Sawyer, H R, McNatty, K P. . Physiology of GDF9 and BMP15 signalling molecules. In Animal reproduction science, 82-83, 447-60. doi:. https://pubmed.ncbi.nlm.nih.gov/15271472/
5. Paulini, Fernanda, Melo, Eduardo O. 2010. The role of oocyte-secreted factors GDF9 and BMP15 in follicular development and oogenesis. In Reproduction in domestic animals = Zuchthygiene, 46, 354-61. doi:10.1111/j.1439-0531.2010.01739.x. https://pubmed.ncbi.nlm.nih.gov/21198974/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.