C57BL/6NCya-Gja1em1flox/Cya
Common Name:
Gja1-flox
Product ID:
S-CKO-02632
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gja1-flox
Strain ID
CKOCMP-14609-Gja1-B6N-VA
Gene Name
Product ID
S-CKO-02632
Gene Alias
Cnx43; Cx43; Cx43alpha1; Cxnk1; Gja-1; Npm1; connexin43
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Gja1em1flox/Cya mice (Catalog S-CKO-02632) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000068581
NCBI RefSeq
NM_010288
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Gja1, encoding Connexin 43 (Cx43), is the primary gap junction protein in mammalian heart ventricles [1,3]. Gap junctions formed by Cx43 are crucial for intercellular communication, facilitating the efficient spread of electrical excitation in cardiac muscle [1]. It also plays roles in various cellular processes like cell-cell contact and is implicated in multiple biological pathways and disease-related processes [1,2,4,5,6]. Genetic models, such as knockout (KO) mouse models, are valuable for studying Gja1's functions.
Gja1-20k, an N-terminal truncated protein isoform of Cx43 generated by internal translation of Gja1 mRNA, is essential for the trafficking of full-length Cx43 hexameric hemichannels to cell-cell contacts [1]. Gja1-20k deficient mice (produced by a M213L substitution in Gja1) suffer from poor electrical coupling between cardiomycytes and arrhythmogenic sudden death 2-4 weeks after birth, highlighting its importance in maintaining normal cardiac electrical rhythm [1]. Additionally, exogenous GJA1-20k expression mimics the effect of ischemic preconditioning in mouse heart and localizes to the mitochondrial outer membrane, inducing a protective form of mitochondrial fission independent of DRP1, preserving ATP production and reducing reactive oxygen species (ROS) under metabolic stress [1].
In conclusion, Gja1 is vital for normal cardiac function, especially in maintaining electrical coupling and rhythm through the function of its isoform Gja1-20k. The study of Gja1-20k deficient mouse models has provided insights into the role of Gja1 in cardiac-related diseases, such as arrhythmias and ischemic-related insults, enhancing our understanding of the underlying mechanisms and potentially guiding the development of new therapeutic strategies.
References:
1. Shimura, Daisuke, Shaw, Robin M. 2022. GJA1-20k and Mitochondrial Dynamics. In Frontiers in physiology, 13, 867358. doi:10.3389/fphys.2022.867358. https://pubmed.ncbi.nlm.nih.gov/35399255/
2. Chen, Kai, Wang, Qi, Liu, Xinxin, Yang, Yinmo, Tian, Xiaodong. 2022. Hypoxic pancreatic cancer derived exosomal miR-30b-5p promotes tumor angiogenesis by inhibiting GJA1 expression. In International journal of biological sciences, 18, 1220-1237. doi:10.7150/ijbs.67675. https://pubmed.ncbi.nlm.nih.gov/35173549/
3. Whisenant, Claire C, Shaw, Robin M. 2022. Internal translation of Gja1 (Connexin43) to produce GJA1-20k: Implications for arrhythmia and ischemic-preconditioning. In Frontiers in physiology, 13, 1058954. doi:10.3389/fphys.2022.1058954. https://pubmed.ncbi.nlm.nih.gov/36569758/
4. Wu, Linqing, Wang, Zengbin, He, Xiaoli, Yu, Huahui, Zhang, Tao. 2022. GJA1 reverses arsenic-induced EMT via modulating MAPK/ERK signaling pathway. In Toxicology and applied pharmacology, 450, 116138. doi:10.1016/j.taap.2022.116138. https://pubmed.ncbi.nlm.nih.gov/35750204/
5. Fournier, Sarah, Clarhaut, Jonathan, Cronier, Laurent, Monvoisin, Arnaud. 2025. GJA1-20k, a Short Isoform of Connexin43, from Its Discovery to Its Potential Implication in Cancer Progression. In Cells, 14, . doi:10.3390/cells14030180. https://pubmed.ncbi.nlm.nih.gov/39936974/
6. Meng, Silu, Fan, Xinran, Zhang, Jianwei, An, Ran, Li, Shuang. 2020. GJA1 Expression and Its Prognostic Value in Cervical Cancer. In BioMed research international, 2020, 8827920. doi:10.1155/2020/8827920. https://pubmed.ncbi.nlm.nih.gov/33299882/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen