C57BL/6JCya-Gclcem1flox/Cya
Common Name:
Gclc-flox
Product ID:
S-CKO-02649
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gclc-flox
Strain ID
CKOCMP-14629-Gclc-B6J-VA
Gene Name
Product ID
S-CKO-02649
Gene Alias
D9Wsu168e; GLCL-H; Ggcs-hs; Glclc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gclcem1flox/Cya mice (Catalog S-CKO-02649) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034905
NCBI RefSeq
NM_010295
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Gclc, also known as glutamate-cysteine ligase catalytic subunit, is a key enzyme in the biosynthesis of glutathione (GSH), a crucial antioxidant that maintains intracellular redox homeostasis [5,7,8,9]. It is involved in pathways such as the NRF2-KEAP1-GCLC pathway, which plays a role in cellular response to oxidative stress and is associated with processes like ferroptosis [1,2,3,4,5,9].
In various disease-related studies, Gclc has been shown to be important. In H1N1 virus-infected nasal mucosal epithelial cells, the virus can induce ferroptosis via the NRF2-KEAP1-GCLC pathway, leading to nasal mucosal epithelial inflammation [1]. In cancer, mechanisms such as NSUN2 lactylation enhancing GCLC-dependent glutathione synthesis and SNORA56-mediated pseudouridylation of 28S rRNA promoting GCLC translation are involved in cancer cell resistance to ferroptosis and cancer proliferation [2,3]. In gastric cancer, ACTL6A upregulates GCLC to protect cells from ferroptosis [4]. In non-small-cell lung cancer, GCLC has a non-canonical role in protecting against ferroptosis by maintaining glutamate homeostasis under cystine starvation [5]. In ischemia-reperfusion induced acute kidney injury, Gclc is a potential marker for injured distal nephron, and its inhibition promotes damage to renal tubular epithelial cells [6]. In T cells, Gclc-deficient T cells cannot meet their increased energy and biosynthetic requirements, affecting T cell effector functions [7]. In age-related cataract, preventing GCLC truncation delays cataract formation [8]. In human cancer cells, SIRT2-regulated GCLC desuccinylation helps cells maintain redox balance against oxidative stress-induced ferroptosis [9]. In pancreatic endocrine progenitor cells, Gclc gene deletion causes a severe diabetes phenotype [10].
In conclusion, Gclc is essential for maintaining redox homeostasis, and its functions are implicated in multiple disease conditions such as viral-induced inflammation, cancer, kidney injury, diabetes, and age-related cataract. Studies using gene-knockout or knockdown models have significantly enhanced our understanding of Gclc's role in these biological processes and diseases, providing potential therapeutic targets for these conditions.
References:
1. Liu, Chengcheng, Wu, Xinhao, Bing, Xin, Zhao, Miaoqing, Xia, Ming. 2023. H1N1 influenza virus infection through NRF2-KEAP1-GCLC pathway induces ferroptosis in nasal mucosal epithelial cells. In Free radical biology & medicine, 204, 226-242. doi:10.1016/j.freeradbiomed.2023.05.004. https://pubmed.ncbi.nlm.nih.gov/37146698/
2. Niu, Kaifeng, Chen, Zixiang, Li, Mengge, Wang, Jiaqi, Zhao, Yongliang. 2024. NSUN2 lactylation drives cancer cell resistance to ferroptosis through enhancing GCLC-dependent glutathione synthesis. In Redox biology, 79, 103479. doi:10.1016/j.redox.2024.103479. https://pubmed.ncbi.nlm.nih.gov/39742570/
3. Xu, Chang, Bian, Zhixuan, Wang, Xinyue, Zhu, Xiaoli, Pan, Qiuhui. 2023. SNORA56-mediated pseudouridylation of 28 S rRNA inhibits ferroptosis and promotes colorectal cancer proliferation by enhancing GCLC translation. In Journal of experimental & clinical cancer research : CR, 42, 331. doi:10.1186/s13046-023-02906-8. https://pubmed.ncbi.nlm.nih.gov/38049865/
4. Yang, Ziqing, Zou, Shaomin, Zhang, Yijing, Lee, Mong-Hong, Fang, Lekun. 2023. ACTL6A protects gastric cancer cells against ferroptosis through induction of glutathione synthesis. In Nature communications, 14, 4193. doi:10.1038/s41467-023-39901-8. https://pubmed.ncbi.nlm.nih.gov/37443154/
5. Kang, Yun Pyo, Mockabee-Macias, Andrea, Jiang, Chang, Harris, Isaac S, DeNicola, Gina M. 2020. Non-canonical Glutamate-Cysteine Ligase Activity Protects against Ferroptosis. In Cell metabolism, 33, 174-189.e7. doi:10.1016/j.cmet.2020.12.007. https://pubmed.ncbi.nlm.nih.gov/33357455/
6. Li, Yinzheng, Ma, Shulin, Wang, Zheng, Zeng, Rui, Yao, Ying. 2024. Gclc as a Marker for Injured Distal Nephron in Ischemia-Reperfusion Induced Acute Kidney Injury. In Journal of inflammation research, 17, 527-540. doi:10.2147/JIR.S451402. https://pubmed.ncbi.nlm.nih.gov/38313210/
7. Mak, Tak W, Grusdat, Melanie, Duncan, Gordon S, Hiller, Karsten, Brenner, Dirk. . Glutathione Primes T Cell Metabolism for Inflammation. In Immunity, 46, 675-689. doi:10.1016/j.immuni.2017.03.019. https://pubmed.ncbi.nlm.nih.gov/28423341/
8. Wei, Zongbo, Hao, Caili, Radeen, Kazi Rafsan, Monnier, Vincent M, Fan, Xingjun. 2024. Prevention of age-related truncation of γ-glutamylcysteine ligase catalytic subunit (GCLC) delays cataract formation. In Science advances, 10, eadl1088. doi:10.1126/sciadv.adl1088. https://pubmed.ncbi.nlm.nih.gov/38669339/
9. Chen, Zixiang, Niu, Kaifeng, Li, Mengge, Wang, Jiaqi, Zhao, Yongliang. 2025. GCLC desuccinylation regulated by oxidative stress protects human cancer cells from ferroptosis. In Cell death and differentiation, , . doi:10.1038/s41418-025-01505-8. https://pubmed.ncbi.nlm.nih.gov/40188196/
10. Davidson, Emily A, Chen, Ying, Singh, Surendra, Thompson, David C, Vasiliou, Vasilis. 2023. Endocrine pancreas-specific Gclc gene deletion causes a severe diabetes phenotype. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.06.13.544855. https://pubmed.ncbi.nlm.nih.gov/37398356/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen