C57BL/6JCya-Gnpatem1flox/Cya
Common Name
Gnpat-flox
Product ID
S-CKO-02699
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-14712-Gnpat-B6J-VA
Status
When using this mouse strain in a publication, please cite “Gnpat-flox Mouse (Catalog S-CKO-02699) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Gnpat-flox
Strain ID
CKOCMP-14712-Gnpat-B6J-VA
Gene Name
Product ID
S-CKO-02699
Gene Alias
DHAPAT, D1Ertd819e
Background
C57BL/6JCya
Gene Full Name
glyceronephosphate O-acyltransferase
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 8 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000034466
NCBI Transcript ID
NM_010322
Target Region
Exon 3~4
Size of Effective Region
~1.7 kb
Overview of Gene Research
Gnpat, also known as glyceronephosphate O-acyltransferase, is an enzyme involved in plasmalogen synthesis, a crucial lipid metabolic pathway [2,3]. Plasmalogens play important roles in various biological processes, and thus Gnpat is of significant biological importance. Genetic models, such as knockout mice, have been valuable in studying its function.
In murine models, Gnpat-knockout (Gnpat-/ -) and hepatocyte-specific Gnpat-knockout mice (Gnpatfl/fl;Alb-Cre) showed no significant difference in serum iron and tissue iron levels compared to wild-type mice. Also, Gnpat deletion did not affect hepcidin expression, suggesting that Gnpat does not play an essential role in systemic iron homeostasis [1]. In adipose-specific knockdown of Gnpat, it recapitulated the effects of Pex16 inactivation on mitochondrial morphology and function in brown and beige adipocytes, indicating its role in regulating adipose thermogenesis through mediating cold-induced mitochondrial fission [2].
In conclusion, Gnpat is mainly involved in lipid-related biological processes, including plasmalogen synthesis, adipose thermogenesis, and potentially lipid metabolism in hepatocarcinogenesis. The study of Gnpat-knockout mouse models has provided insights into its non-essential role in systemic iron homeostasis and its function in adipose-related thermogenesis. These findings contribute to understanding the underlying mechanisms of related diseases and biological processes.
References：
1. An, Peng, Wang, Jiaming, Wang, Hao, Min, Junxia, Wang, Fudi. 2020. Gnpat does not play an essential role in systemic iron homeostasis in murine model. In Journal of cellular and molecular medicine, 24, 4118-4126. doi:10.1111/jcmm.15068. https://pubmed.ncbi.nlm.nih.gov/32108988/
2. Park, Hongsuk, He, Anyuan, Tan, Min, Funai, Katsuhiko, Lodhi, Irfan J. 2019. Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission. In The Journal of clinical investigation, 129, 694-711. doi:10.1172/JCI120606. https://pubmed.ncbi.nlm.nih.gov/30511960/
3. Bertolesi, Gabriel E, Chilije, Maxwell F J, Li, Victoria, Zaremberg, Vanina, McFarlane, Sarah. . Differential Eye Expression of Xenopus Acyltransferase Gnpat and Its Biochemical Characterization Shed Light on Lipid-Associated Ocular Pathologies. In Investigative ophthalmology & visual science, 64, 17. doi:10.1167/iovs.64.5.17. https://pubmed.ncbi.nlm.nih.gov/37204785/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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