Gnpat, also known as glyceronephosphate O-acyltransferase, is an enzyme involved in plasmalogen synthesis, a crucial lipid metabolic pathway [2,3]. Plasmalogens play important roles in various biological processes, and thus Gnpat is of significant biological importance. Genetic models, such as knockout mice, have been valuable in studying its function.

In murine models, Gnpat-knockout (Gnpat-/ -) and hepatocyte-specific Gnpat-knockout mice (Gnpatfl/fl;Alb-Cre) showed no significant difference in serum iron and tissue iron levels compared to wild-type mice. Also, Gnpat deletion did not affect hepcidin expression, suggesting that Gnpat does not play an essential role in systemic iron homeostasis [1]. In adipose-specific knockdown of Gnpat, it recapitulated the effects of Pex16 inactivation on mitochondrial morphology and function in brown and beige adipocytes, indicating its role in regulating adipose thermogenesis through mediating cold-induced mitochondrial fission [2].

In conclusion, Gnpat is mainly involved in lipid-related biological processes, including plasmalogen synthesis, adipose thermogenesis, and potentially lipid metabolism in hepatocarcinogenesis. The study of Gnpat-knockout mouse models has provided insights into its non-essential role in systemic iron homeostasis and its function in adipose-related thermogenesis. These findings contribute to understanding the underlying mechanisms of related diseases and biological processes.