C57BL/6JCya-Lancl1em1flox/Cya
Common Name:
Lancl1-flox
Product ID:
S-CKO-02729
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lancl1-flox
Strain ID
CKOCMP-14768-Lancl1-B6J-VA
Gene Name
Product ID
S-CKO-02729
Gene Alias
Gpr69; Gpr69a; p40
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lancl1em1flox/Cya mice (Catalog S-CKO-02729) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113979
NCBI RefSeq
NM_001190985
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Lancl1, or Lanthionine synthetase C-like protein 1, is an antioxidant-related protein with multiple functions. It has been implicated in various pathways, such as those related to oxidative stress regulation, and is biologically important in multiple tissues. Genetic models, especially knockout (KO) mouse models, can help uncover its specific functions [1].
In a transgenic SOD1G93A mouse model of amyotrophic lateral sclerosis (ALS), CNS-specific expression of Lancl1 transgene extended lifespan, delayed disease onset, decelerated symptomatic progression, and improved motor performance. Conversely, CNS-specific deletion of Lancl1 led to neurodegenerative phenotypes, including motor neuron loss, neuroinflammation, and oxidative damage. This indicates Lancl1 is a positive regulator of AKT activity, regulating neuronal survival [1].
In an in vitro model of ischemia mimicked by oxygen and glucose deprivation (OGD) in neuronal HT22 cells, overexpression of Lancl1 by lentivirus transfection preserved cell viability, reduced lactate dehydrogenase release, and attenuated apoptosis. Lancl1 protected against OGD through activating the Akt-PGC-1α-Sirt3 pathway [2].
In prostate cancer cells, suppression of Lancl1 by siRNA increased cancer cell apoptosis, suggesting Lancl1 promotes cell proliferation and protects cells from oxidative stress via suppression of the JNK pathway [3].
In conclusion, Lancl1 plays essential roles in protecting neurons from oxidative stress-related injuries and in the regulation of cell survival in neurodegenerative diseases like ALS and in cancer. The use of KO and transgenic mouse models, as well as in vitro models, has been crucial in revealing these functions, providing potential therapeutic targets for these disease areas.
References:
1. Tan, Honglin, Chen, Mina, Pang, Dejiang, Worley, Paul F, Xiao, Bo. 2019. LanCL1 promotes motor neuron survival and extends the lifespan of amyotrophic lateral sclerosis mice. In Cell death and differentiation, 27, 1369-1382. doi:10.1038/s41418-019-0422-6. https://pubmed.ncbi.nlm.nih.gov/31570855/
2. Xie, Zhen, Cao, Bing-Qing, Wang, Tao, Gao, Wen-Jie, Hui, Hao. 2018. LanCL1 attenuates ischemia-induced oxidative stress by Sirt3-mediated preservation of mitochondrial function. In Brain research bulletin, 142, 216-223. doi:10.1016/j.brainresbull.2018.07.017. https://pubmed.ncbi.nlm.nih.gov/30075199/
3. Wang, Jianqing, Xiao, Qianyi, Chen, Xu, Fan, Caibin, Ding, Guanxiong. 2018. LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway. In Cell death & disease, 9, 197. doi:10.1038/s41419-017-0207-0. https://pubmed.ncbi.nlm.nih.gov/29416001/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen