C57BL/6JCya-Gtf2h4em1flox/Cya
Common Name:
Gtf2h4-flox
Product ID:
S-CKO-02789
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gtf2h4-flox
Strain ID
CKOCMP-14885-Gtf2h4-B6J-VA
Gene Name
Product ID
S-CKO-02789
Gene Alias
BTF2 p52; p52
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gtf2h4em1flox/Cya mice (Catalog S-CKO-02789) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001565
NCBI RefSeq
NM_010364
Target Region
Exon 4~11
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Gtf2h4, or general transcription factor IIH subunit 4, is involved in transcription-dependent DNA repair mechanisms [2]. It is part of the major histocompatibility complex (MHC) class II region and may play a role in the immune system [3].
In ischemic diseases, Gtf2h4 promotes partial endothelial-to-mesenchymal transition (EndMT) and angiogenesis after ischemic injury. It activates NF-κB through NCOA3 phosphorylation at serine 1330, which enhances the interaction between NCOA3 and p65, thus activating the NF-κB/Snail signaling axis during partial EndMT [1]. Genetic variant rs114596632 in Gtf2h4 is associated with lung cancer risk, as the T allele is related to decreased DNA repair capacity phenotype and decreased mRNA expression levels [4]. In prostate cancer, Gtf2h4 is highly expressed, and high expression is associated with a higher biochemical recurrence rate, indicating a poor prognosis [5].
In summary, Gtf2h4 has significant functions in processes like EndMT and angiogenesis in ischemic diseases, and is associated with the risk and prognosis of diseases such as lung cancer and prostate cancer. Studies on Gtf2h4 contribute to understanding disease mechanisms and may offer potential therapeutic targets for hypoxic/ischemic diseases, lung cancer, and prostate cancer.
References：
1. Fang, Zheyan, Zhao, Gang, Zhao, Shuang, Ge, Junbo, Li, Hua. 2024. GTF2H4 regulates partial EndMT via NF-κB activation through NCOA3 phosphorylation in ischemic diseases. In Innovation (Cambridge (Mass.)), 5, 100565. doi:10.1016/j.xinn.2024.100565. https://pubmed.ncbi.nlm.nih.gov/38379791/
2. Porter, Louise F, Saptarshi, Neil, Fang, Yongxiang, Chavali, Venkata R M, Paraoan, Luminita. 2019. Whole-genome methylation profiling of the retinal pigment epithelium of individuals with age-related macular degeneration reveals differential methylation of the SKI, GTF2H4, and TNXB genes. In Clinical epigenetics, 11, 6. doi:10.1186/s13148-019-0608-2. https://pubmed.ncbi.nlm.nih.gov/30642396/
3. Kim, J Y, Kim, J-H, Bae, J S, Park, C-S, Shin, H D. 2012. Lack of association between GTF2H4 genetic variants and AERD development and FEV1 decline by aspirin provocation. In International journal of immunogenetics, 39, 486-91. doi:10.1111/j.1744-313X.2012.01118.x. https://pubmed.ncbi.nlm.nih.gov/22524621/
4. Wang, Meilin, Liu, Hongliang, Liu, Zhensheng, Amos, Christopher I, Wei, Qingyi. 2016. Genetic variant in DNA repair gene GTF2H4 is associated with lung cancer risk: a large-scale analysis of six published GWAS datasets in the TRICL consortium. In Carcinogenesis, 37, 888-896. doi:10.1093/carcin/bgw070. https://pubmed.ncbi.nlm.nih.gov/27288692/
5. Li, J C, Zhu, X J, Ye, J H, Luo, D H, Zhong, W D. . [Bioinformatics-based analysis of the effect of general Transcription Factor IIH on prognosis of Prostate cancer]. In Zhonghua yi xue za zhi, 104, 1410-1417. doi:10.3760/cma.j.cn112137-20231228-01504. https://pubmed.ncbi.nlm.nih.gov/38644292/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen