C57BL/6JCya-Gys1em1flox/Cya
Common Name:
Gys1-flox
Product ID:
S-CKO-02805
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gys1-flox
Strain ID
CKOCMP-14936-Gys1-B6J-VA
Gene Name
Product ID
S-CKO-02805
Gene Alias
Gys3; MGS
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gys1em1flox/Cya mice (Catalog S-CKO-02805) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003964
NCBI RefSeq
NM_030678
Target Region
Exon 6~8
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Gys1, glycogen synthase 1, is a central enzyme for glycogen synthesis [4]. It is involved in processes like gluconeogenesis and glycogenesis, and plays a crucial role in maintaining glycogen metabolism, which is essential for energy storage and various biological functions [5]. In muscle, it works with glycogenin-1 (GYG1) to initiate and extend glycogen chains [6].
In mouse models, downregulating Gys1 has shown therapeutic potential in several diseases. In Lafora disease mouse models, Gys1-ASO treatment decreased Gys1 protein levels, glycogen aggregation, and epileptiform discharges, halting disease progression [1]. Similarly, in adult polyglucosan body disease mouse models, knocking out Gys1 improved lifespan, morphology, and neuromuscular function, reducing polyglucosan body accumulation and gliosis [2]. In clear cell renal carcinoma (ccRCC), silencing Gys1 suppressed tumor growth by activating the NF-κB pathway [3]. In zebrafish, gys1 knockout led to reduced glycogen reserve in ovaries and embryos, indicating its role in maternal glycogen reserve for embryonic development [4].
In conclusion, Gys1 is essential for glycogen metabolism. Studies using gene knockout or knockdown in mouse models and zebrafish have revealed its significance in diseases such as Lafora disease, adult polyglucosan body disease, and ccRCC, as well as in embryonic development. These findings provide potential therapeutic targets for related diseases and a better understanding of biological processes related to glycogen metabolism.
References:
1. Donohue, Katherine J, Fitzsimmons, Bethany, Bruntz, Ronald C, Kordasiewisz, Holly B, Gentry, Matthew S. 2023. Gys1 Antisense Therapy Prevents Disease-Driving Aggregates and Epileptiform Discharges in a Lafora Disease Mouse Model. In Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 20, 1808-1819. doi:10.1007/s13311-023-01434-9. https://pubmed.ncbi.nlm.nih.gov/37700152/
2. Chown, Erin E, Wang, Peixiang, Zhao, Xiaochu, Pederson, Bartholomew A, Minassian, Berge A. 2020. GYS1 or PPP1R3C deficiency rescues murine adult polyglucosan body disease. In Annals of clinical and translational neurology, 7, 2186-2198. doi:10.1002/acn3.51211. https://pubmed.ncbi.nlm.nih.gov/33034425/
3. Chen, Shi-Lu, Huang, Qun-Sheng, Huang, Yu-Hua, Guan, Xin-Yuan, Yun, Jing-Ping. 2020. GYS1 induces glycogen accumulation and promotes tumor progression via the NF-κB pathway in Clear Cell Renal Carcinoma. In Theranostics, 10, 9186-9199. doi:10.7150/thno.46825. https://pubmed.ncbi.nlm.nih.gov/32802186/
4. Chen, Jie, Ji, Xiao, Gao, Jing, Huang, Jiao, Ren, Jianfeng. 2024. gys1 regulates maternal glycogen reserve essential for embryonic development in zebrafish. In Heliyon, 10, e31149. doi:10.1016/j.heliyon.2024.e31149. https://pubmed.ncbi.nlm.nih.gov/38803914/
5. Sadiku, Pranvera, Willson, Joseph A, Ryan, Eilise M, Whyte, Moira K B, Walmsley, Sarah R. 2020. Neutrophils Fuel Effective Immune Responses through Gluconeogenesis and Glycogenesis. In Cell metabolism, 33, 411-423.e4. doi:10.1016/j.cmet.2020.11.016. https://pubmed.ncbi.nlm.nih.gov/33306983/
6. Fastman, Nathan M, Liu, Yuxi, Ramanan, Vyas, Morgans, David, Tzitzilonis, Christos. . The structural mechanism of human glycogen synthesis by the GYS1-GYG1 complex. In Cell reports, 40, 111041. doi:10.1016/j.celrep.2022.111041. https://pubmed.ncbi.nlm.nih.gov/35793618/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen