C57BL/6JCya-Gzmbem1flox/Cya
Common Name:
Gzmb-flox
Product ID:
S-CKO-02807
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gzmb-flox
Strain ID
CKOCMP-14939-Gzmb-B6J-VA
Gene Name
Product ID
S-CKO-02807
Gene Alias
CCP-1/C11; CCP1; Ctla-1; Ctla1; GZB
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gzmbem1flox/Cya mice (Catalog S-CKO-02807) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015581
NCBI RefSeq
NM_013542
Target Region
Exon 2~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Gzmb, also known as Granzyme B, is a serine protease found in the cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells. It plays a crucial role in inducing apoptotic changes of target cells, and is involved in immune-related processes such as immune suppression and regulation of T-cell function [1,4].
In research, it has been found that in generalized vitiligo patients, reduced GZMB transcripts lead to a decrease in the suppressive capacity of regulatory T cells, suggesting its importance in the pathogenesis of this autoimmune disease [1]. In non-segmental vitiligo, certain GZMB polymorphisms are associated with the development of the disease, indicating a genetic link [4]. In skin cutaneous melanoma, high levels of GZMB expression are associated with favorable prognosis, making it a potential prognostic biomarker [2]. Also, disruption of GZMB in human CD8+ T cells results in reduced suppressive function, highlighting its role in immune regulation [3].
In conclusion, Gzmb is essential for immune-related functions, especially in the regulation of T-cell-mediated immune responses. Studies on Gzmb, including those using gene-knockout models (implied by functional disruption studies), have provided insights into its role in autoimmune diseases like vitiligo and in cancer prognosis, contributing to a better understanding of disease mechanisms and potential therapeutic targets.
References:
1. Giri, Prashant S, Bharti, Ankit H, Dwivedi, Mitesh. 2022. Decreased GZMB, NRP1, ITPR1, and SERPINB9 Transcripts Lead to Reduced Regulatory T Cells Suppressive Capacity in Generalized Vitiligo Patients. In Journal of immunology research, 2022, 3426717. doi:10.1155/2022/3426717. https://pubmed.ncbi.nlm.nih.gov/36157881/
2. Liang, Zhuoshuai, Pan, Lingfeng, Shi, Jikang, Zhang, Lianbo. 2022. C1QA, C1QB, and GZMB are novel prognostic biomarkers of skin cutaneous melanoma relating tumor microenvironment. In Scientific reports, 12, 20460. doi:10.1038/s41598-022-24353-9. https://pubmed.ncbi.nlm.nih.gov/36443341/
3. Vemulawada, Chakrapani, Renavikar, Pranav S, Crawford, Michael P, Steward-Tharp, Scott, Karandikar, Nitin J. . Disruption of IFNγ, GZMB, PRF1, or LYST Results in Reduced Suppressive Function in Human CD8+ T Cells. In Journal of immunology (Baltimore, Md. : 1950), 212, 1722-1732. doi:10.4049/jimmunol.2300388. https://pubmed.ncbi.nlm.nih.gov/38607279/
4. Jeong, Ki-Heon, Kim, Su Kang, Seo, Jong-Kil, Shin, Min Kyung, Lee, Mu-Hyoung. 2021. Association of GZMB polymorphisms and susceptibility to non-segmental vitiligo in a Korean population. In Scientific reports, 11, 397. doi:10.1038/s41598-020-79705-0. https://pubmed.ncbi.nlm.nih.gov/33431938/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen