C57BL/6JCya-H2-Ab1em1flox/Cya
Common Name:
H2-Ab1-flox
Product ID:
S-CKO-02815
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
H2-Ab1-flox
Strain ID
CKOCMP-14961-H2-Ab1-B6J-VA
Gene Name
Product ID
S-CKO-02815
Gene Alias
Abeta; H-2Ab; H2-Ab; I-Abeta; IAb; Ia-2; Ia2; Rmcs1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-H2-Ab1em1flox/Cya mice (Catalog S-CKO-02815) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040828
NCBI RefSeq
NM_207105
Target Region
Exon 1
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
H2-Ab1, also known as histocompatibility 2, class II antigen A, beta 1, is a gene encoding the beta chain of the Class II heterodimer H2-A, which is a major histocompatibility complex (MHC) class II protein. MHC class II proteins are involved in the immune response by presenting antigens to CD4+ T cells, thus playing a crucial role in activating the adaptive immune system [4].
In a study on pulmonary arterial hypertension (PAH), H2-Ab1 was identified as a key gene. Knocking down H2-Ab1 in mouse pulmonary artery endothelial cells (PAECs) inhibited cell viability, adhesion, migration, and angiogenesis, while promoting apoptosis, indicating its role in PAH vascular remodeling [1].
In allergic rhinitis, double knockout of H2-Ab1 and H2-Eb1 in mice reduced the susceptibility to the disease, with fewer histological changes in nasal mucosa, reduced eosinophilic granulocytes and mast cells, and altered Th1/Th2 cell factor balance [2].
In colitis mouse models, disruption of H2-Ab1 in intestinal epithelial cells (IECs) led to less severe DSS-or T-cell transfer-induced colitis, but also reduced bacterial clearance in Citrobacter rodentium-induced colitis due to decreased IgA binding to bacteria [3].
In renal fibrosis models, global or renal tubule-specific ablation of H2-Ab1 alleviated fibrosis, with changes in renal CD4+ T cell subsets and profibrotic gene expression [5].
In conclusion, H2-Ab1 is essential for the normal function of the immune response through its role in antigen presentation. Gene knockout studies in mice have revealed its significance in multiple disease conditions such as PAH, allergic rhinitis, colitis, and renal fibrosis. These findings contribute to understanding the pathogenesis of these diseases and may provide potential therapeutic targets related to H2-Ab1-mediated immune regulation.
References:
1. Wang, Guowen, Wang, Zhuoyan. 2024. Investigation into the role of H2-Ab1 in vascular remodeling in pulmonary arterial hypertension via Bioinformatics. In BMC pulmonary medicine, 24, 342. doi:10.1186/s12890-024-03156-w. https://pubmed.ncbi.nlm.nih.gov/39010027/
2. Tang, Zhiyuan, Wang, Yan, Lv, Liang, Li, Linge, Zhang, Hua. 2018. Mice with double knockout of H2-Eb1 and H2-Ab1 exhibit reduced susceptibility to allergic rhinitis. In PloS one, 13, e0206122. doi:10.1371/journal.pone.0206122. https://pubmed.ncbi.nlm.nih.gov/30372475/
3. Jamwal, Deepa R, Laubitz, Daniel, Harrison, Christy A, Ghishan, Fayez K, Kiela, Pawel R. 2020. Intestinal Epithelial Expression of MHCII Determines Severity of Chemical, T-Cell-Induced, and Infectious Colitis in Mice. In Gastroenterology, 159, 1342-1356.e6. doi:10.1053/j.gastro.2020.06.049. https://pubmed.ncbi.nlm.nih.gov/32589883/
4. Logunova, Nadezhda, Korotetskaya, Maria, Polshakov, Vladimir, Apt, Alexander. 2015. The QTL within the H2 Complex Involved in the Control of Tuberculosis Infection in Mice Is the Classical Class II H2-Ab1 Gene. In PLoS genetics, 11, e1005672. doi:10.1371/journal.pgen.1005672. https://pubmed.ncbi.nlm.nih.gov/26618355/
5. Zhou, Yunfeng, Luo, Zhaokang, Liao, Chenghui, Guan, Youfei, Deng, Tuo. 2021. MHC class II in renal tubules plays an essential role in renal fibrosis. In Cellular & molecular immunology, 18, 2530-2540. doi:10.1038/s41423-021-00763-z. https://pubmed.ncbi.nlm.nih.gov/34556823/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen