C57BL/6JCya-Hap1em1flox/Cya
Common Name:
Hap1-flox
Product ID:
S-CKO-02832
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hap1-flox
Strain ID
CKOCMP-15114-Hap1-B6J-VA
Gene Name
Product ID
S-CKO-02832
Gene Alias
HAP-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hap1em1flox/Cya mice (Catalog S-CKO-02832) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000138603
NCBI RefSeq
NM_177981
Target Region
Exon 2~10
Size of Effective Region
~4.7 kb
Detailed Document
Overview of Gene Research
Hap1, short for huntingtin-associated protein 1, is significantly involved in intracellular trafficking. It binds to microtubule-dependent transporters for anterograde or retrograde transport, and associates with various organelles and membrane receptors. Hap1 is enriched in neurons, and its function may be related to the selective neuropathology in Huntington's disease. Hap1-related research models, especially cell-based models, are valuable for studying its functions [1,2].
Hap1 knockout (KO) studies have revealed multiple functions. In neurons, Hap1 KO abolishes BDNF and TrkB internalization, impairs TrkB downstream signalling pathways like ERK, Akt and PLCγ-1, and affects the proliferation of cerebellar granule cells, suggesting its key role in BDNF and receptor endocytosis, and in promoting neuronal survival and proliferation [3]. In acute lymphoblastic leukemia (ALL), knocking down Hap1 confers l-asparaginase resistance by downregulating the calpain-1-Bid-caspase-3/12 pathway, indicating Hap1 as a potential biomarker for l-asparaginase-resistant ALL [4]. In a pentylenetetrazole rat model of epilepsy, disrupting the HAP1/14-3-3 complex decreases the strength of GABAAR-mediated inhibitory synaptic transmission, suggesting it as a possible drug target for epilepsy [5]. In Angelman syndrome (AS) mouse models, knocking down HAP1 alleviated aberrant autophagy in primary neurons, and autophagy inhibition in AS mice partially alleviated a social interaction deficit [6].
In conclusion, Hap1 plays essential roles in multiple biological processes including intracellular trafficking, endocytosis of BDNF and its receptors, regulation of autophagy, and influencing signalling pathways related to cell survival, proliferation, and apoptosis. The gene knockout studies in mouse models have provided crucial insights into Hap1's functions in diseases such as Huntington's disease, ALL, epilepsy, and Angelman syndrome, which may contribute to the development of new therapeutic strategies for these conditions.
References:
1. Li, Xiao-Jiang, Li, Shi-Hua. . HAP1 and intracellular trafficking. In Trends in pharmacological sciences, 26, 1-3. doi:. https://pubmed.ncbi.nlm.nih.gov/15629196/
2. Rong, Juan, Li, Shi-Hua, Li, Xiao-Jiang. . Regulation of intracellular HAP1 trafficking. In Journal of neuroscience research, 85, 3025-9. doi:. https://pubmed.ncbi.nlm.nih.gov/17474105/
3. Lim, Yoon, Wu, Linda Lin-Yan, Chen, Si, Li, Xiao-Jiang, Zhou, Xin-Fu. 2017. HAP1 Is Required for Endocytosis and Signalling of BDNF and Its Receptors in Neurons. In Molecular neurobiology, 55, 1815-1830. doi:10.1007/s12035-016-0379-0. https://pubmed.ncbi.nlm.nih.gov/28083816/
4. Lee, Jung Kwon, Kang, SungMyung, Wang, Xidi, Wang, Jinghua, Lee, Ki-Young. 2019. HAP1 loss confers l-asparaginase resistance in ALL by downregulating the calpain-1-Bid-caspase-3/12 pathway. In Blood, 133, 2222-2232. doi:10.1182/blood-2018-12-890236. https://pubmed.ncbi.nlm.nih.gov/30819925/
5. Wen, Yuetao, Zhang, Guangliang, Liu, Lin, Chen, Yangmei, Li, Rong. 2022. HAP1 interacts with 14-3-3 to regulate epileptic seizure via GABAAR-mediated inhibitory synaptic transmission in pentylenetetrazole rat model. In Neuroscience research, 182, 7-14. doi:10.1016/j.neures.2022.05.006. https://pubmed.ncbi.nlm.nih.gov/35609730/
6. Wang, Tingting, Wang, Jingyu, Wang, Jie, Xiong, Zhi-Qi, Liao, Lujian. 2019. HAP1 is an in vivo UBE3A target that augments autophagy in a mouse model of Angelman syndrome. In Neurobiology of disease, 132, 104585. doi:10.1016/j.nbd.2019.104585. https://pubmed.ncbi.nlm.nih.gov/31445164/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen