C57BL/6JCya-Herc2em1flox/Cya
Common Name
Herc2-flox
Product ID
S-CKO-02864
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-15204-Herc2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Herc2-flox Mouse (Catalog S-CKO-02864) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Herc2-flox
Strain ID
CKOCMP-15204-Herc2-B6J-VA
Gene Name
Product ID
S-CKO-02864
Gene Alias
rjs, jdf2, D15F32S1h, mKIAA0393, D7H15F32S1, D7H15F37S1
Background
C57BL/6JCya
Gene Full Name
HECT and RLD domain containing E3 ubiquitin protein ligase 2
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 7 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000164095
NCBI Transcript ID
NM_001360080
Target Region
Exon 11~12
Size of Effective Region
~2.1 kb
Overview of Gene Research
HERC2, also known as HECT domain and RCC1-like domain 2, is a giant E3 ubiquitin protein ligase. It is involved in multiple cellular processes such as iron homeostasis, inflammation-related cancer development, and DNA damage response. It participates in pathways like the STAT3 pathway in hepatocellular carcinoma and is associated with controlling the levels of proteins related to iron metabolism [1-3]. Genetic models, especially knockout mouse models, are valuable for studying its functions.
In hepatocyte-specific HERC2-knockout mice, hepatic PD-L1 expression was limited and HCC progression was ameliorated, indicating that HERC2 promotes inflammation-driven cancer stemness and immune evasion in HCC by activating the STAT3 pathway [1]. In liver-specific HERC2-deficient mice under APAP challenge, more severe liver damage was observed, suggesting HERC2 plays a protective role against drug-induced liver injury by regulating β-catenin-regulated CYP2E1 expression [2].
In conclusion, HERC2 is essential in regulating various biological processes and is involved in multiple disease conditions, including hepatocellular carcinoma and drug-induced liver injury. The use of HERC2 knockout mouse models has significantly contributed to understanding its role in these disease areas, highlighting its potential as a therapeutic target.
References：
1. Liu, Yunzhi, Xu, Qishan, Deng, Fan, Chen, Qingyun, Zuo, Daming. 2023. HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway. In Journal of experimental & clinical cancer research : CR, 42, 38. doi:10.1186/s13046-023-02609-0. https://pubmed.ncbi.nlm.nih.gov/36721234/
2. Liu, Yunzhi, Xu, Qishan, Liu, Yan, Chen, Qingyun, Zuo, Daming. 2024. Hepatocyte-Targeted Lipid Nanoparticle Delivery of HERC2 Plasmid Controls Drug-Induced Hepatotoxicity by Limiting β-Catenin-Regulated CYP2E1 Expression. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2401633. doi:10.1002/advs.202401633. https://pubmed.ncbi.nlm.nih.gov/39440550/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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