C57BL/6JCya-Foxd1em1flox/Cya
Common Name:
Foxd1-flox
Product ID:
S-CKO-02881
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Foxd1-flox
Strain ID
CKOCMP-15229-Foxd1-B6J-VA
Gene Name
Product ID
S-CKO-02881
Gene Alias
BF-2; FREAC4; Hfh10; Hfhbf2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Foxd1em1flox/Cya mice (Catalog S-CKO-02881) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105098
NCBI RefSeq
NM_008242
Target Region
Exon 1
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Foxd1, a member of the FOX transcription factor family, is crucial for various biological processes. It has been associated with cell reprogramming and is involved in key developmental events such as kidney and retina development, as well as embryo implantation [3,9]. Dysregulation of Foxd1 has been linked to different pathologies, making it a potential diagnostic biomarker and therapeutic target [9].
In cancer research, Foxd1 has shown oncogenic properties. In pancreatic cancer, its overexpression promotes cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis, while knockdown inhibits these functions [1]. In breast cancer, highly expressed Foxd1 in primary tissues is associated with increased circulating tumor cells (CTCs), and overexpressing Foxd1 enhances BC cell migration, CTC formation, and metastasis via an ERK1/2-related signaling cascade [2]. In uveal melanoma, Foxd1 expression is negatively correlated with patient survival, and in vitro studies suggest it may promote tumor growth and invasion [3]. In head and neck squamous cell carcinoma, Foxd1 promotes cell proliferation by blocking cellular senescence and apoptosis through the p21/CDK2/Rb signaling pathway [5]. In prostate cancer, Foxd1 is upregulated, and its knockdown inhibits cell viability, migration, and invasion [6]. A meta-analysis also indicates that high Foxd1 expression is linked to poor overall and disease-free survival, as well as higher TNM stage in various cancers [7]. In clear cell renal cell carcinoma, knockout of Foxd1 in 786-O cells reduces cell growth, alters mitochondrial metabolism and glycolysis, and extends the G2/M phase of the cell cycle, showing its role in regulating cell division [8]. In oral squamous cell carcinoma, the lncRNA FOXD1-AS1 upregulates Foxd1 to promote cancer progression [4].
In conclusion, Foxd1 is essential for normal development and is involved in multiple biological processes. Its dysregulation contributes to the development and progression of various cancers. The use of gene knockout (KO) and conditional knockout (CKO) mouse models, as well as in vitro functional studies, has been crucial in revealing these roles, providing potential targets for cancer treatment and prognosis evaluation.
References:
1. Cai, Kun, Chen, Shiyu, Zhu, Changhao, He, Zhiwei, Sun, Chengyi. 2022. FOXD1 facilitates pancreatic cancer cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis. In Cell death & disease, 13, 765. doi:10.1038/s41419-022-05213-w. https://pubmed.ncbi.nlm.nih.gov/36057597/
2. Long, Yufei, Chong, Tuotuo, Lyu, Xiaoming, Chen, Ceshi, Li, Xin. 2022. FOXD1-dependent RalA-ANXA2-Src complex promotes CTC formation in breast cancer. In Journal of experimental & clinical cancer research : CR, 41, 301. doi:10.1186/s13046-022-02504-0. https://pubmed.ncbi.nlm.nih.gov/36229838/
3. Luo, Yang, Ni, Renhao, Jin, Xiaojun, Yang, Lu, Zhu, Yabin. 2023. FOXD1 expression-based prognostic model for uveal melanoma. In Heliyon, 9, e21333. doi:10.1016/j.heliyon.2023.e21333. https://pubmed.ncbi.nlm.nih.gov/38027647/
4. Ma, Yuxin, Han, Jingchao, Luo, Xi. 2021. FOXD1-AS1 upregulates FOXD1 to promote oral squamous cell carcinoma progression. In Oral diseases, 29, 604-614. doi:10.1111/odi.14002. https://pubmed.ncbi.nlm.nih.gov/34403535/
5. Wu, Tong, Yang, Zhongyuan, Chen, Weichao, Song, Ming, Yang, Ankui. 2023. miR-30e-5p-mediated FOXD1 promotes cell proliferation by blocking cellular senescence and apoptosis through p21/CDK2/Rb signaling in head and neck carcinoma. In Cell death discovery, 9, 295. doi:10.1038/s41420-023-01571-2. https://pubmed.ncbi.nlm.nih.gov/37563111/
6. Huang, Yuanshe, Zhang, Lai, Liu, Tianlei, Liang, E. 2023. LMNB1 targets FOXD1 to promote progression of prostate cancer. In Experimental and therapeutic medicine, 26, 513. doi:10.3892/etm.2023.12212. https://pubmed.ncbi.nlm.nih.gov/37840569/
7. Liu, Xiaohan, Min, Shengyun, Zhang, Qin, Wang, Nanye, Zhou, Bin. 2024. Prognostic and clinicopathological significance of FOXD1 in various cancers: a meta and bioinformation analysis. In Future science OA, 10, FSO901. doi:10.2144/fsoa-2023-0085. https://pubmed.ncbi.nlm.nih.gov/38827805/
8. Bond, Kyle H, Fetting, Jennifer L, Lary, Christine W, Emery, Ivette F, Oxburgh, Leif. 2021. FOXD1 regulates cell division in clear cell renal cell carcinoma. In BMC cancer, 21, 312. doi:10.1186/s12885-021-07957-8. https://pubmed.ncbi.nlm.nih.gov/33761914/
9. Quintero-Ronderos, Paula, Laissue, Paul. 2018. The multisystemic functions of FOXD1 in development and disease. In Journal of molecular medicine (Berlin, Germany), 96, 725-739. doi:10.1007/s00109-018-1665-2. https://pubmed.ncbi.nlm.nih.gov/29959475/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen