C57BL/6JCya-Hif1aem1flox/Cya
Common Name:
Hif1a-flox
Product ID:
S-CKO-02890
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Hif1a-flox
Strain ID
CKOCMP-15251-Hif1a-B6J-VA
Gene Name
Product ID
S-CKO-02890
Gene Alias
HIF-1-alpha; HIF1-alpha; HIF1alpha; MOP1; bHLHe78
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hif1aem1flox/Cya mice (Catalog S-CKO-02890) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021530
NCBI RefSeq
NM_010431
Target Region
Exon 2~4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Hif1a, also known as hypoxia inducible factor 1 subunit alpha, is a crucial transcription factor responsive to low oxygen availability. It activates the transcription of genes involved in angiogenesis, cell survival, glucose metabolism, and invasion, playing essential roles in mammalian development, physiology, and disease pathogenesis [4,5].
In endothelial cell-specific atg7 knockout (atg7 KO) mice, ablation of Atg7 upregulated Stat1, which suppressed Hif1a expression, inhibiting ischemia-induced angiogenesis, blood flow reperfusion recovery [3]. In colorectal cancer, USP51 forms a positive feed-forward loop with Hif1a, promoting cancer stemness and chemoresistance [2]. In non-small cell lung cancer, lncRNA HIF1A-As2 epigenetically activates MYC, and together they form a double-positive feedback loop to promote cell proliferation and metastasis [1].
In conclusion, Hif1a is essential in regulating hypoxia-related biological processes. Studies using gene knockout models, like atg7 KO mice, have revealed its significance in angiogenesis, cancer stemness, chemoresistance, and tumor cell proliferation and metastasis, providing insights into potential therapeutic strategies for cardiovascular diseases and cancers.
References：
1. Yang, Kaixin, Zhang, Wenyang, Zhong, Linghui, Garofalo, Michela, Shi, Lei. 2023. Long non-coding RNA HIF1A-As2 and MYC form a double-positive feedback loop to promote cell proliferation and metastasis in KRAS-driven non-small cell lung cancer. In Cell death and differentiation, 30, 1533-1549. doi:10.1038/s41418-023-01160-x. https://pubmed.ncbi.nlm.nih.gov/37041291/
2. Mu, Mingchao, Zhang, Qin, Li, Jing, Sun, Xuejun, Yu, Junhui. 2023. USP51 facilitates colorectal cancer stemness and chemoresistance by forming a positive feed-forward loop with HIF1A. In Cell death and differentiation, 30, 2393-2407. doi:10.1038/s41418-023-01228-8. https://pubmed.ncbi.nlm.nih.gov/37816999/
3. Yao, Hongmin, Li, Jian, Liu, Zhixue, Mu, Jing, Xie, Zhonglin. 2022. Ablation of endothelial Atg7 inhibits ischemia-induced angiogenesis by upregulating Stat1 that suppresses Hif1a expression. In Autophagy, 19, 1491-1511. doi:10.1080/15548627.2022.2139920. https://pubmed.ncbi.nlm.nih.gov/36300763/
4. Semenza, Gregg L. . Targeting HIF-1 for cancer therapy. In Nature reviews. Cancer, 3, 721-32. doi:. https://pubmed.ncbi.nlm.nih.gov/13130303/
5. Semenza, G L. . HIF-1 and mechanisms of hypoxia sensing. In Current opinion in cell biology, 13, 167-71. doi:. https://pubmed.ncbi.nlm.nih.gov/11248550/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen