C57BL/6JCya-Hnrnpcem1flox/Cya
Common Name:
Hnrnpc-flox
Product ID:
S-CKO-02923
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hnrnpc-flox
Strain ID
CKOCMP-15381-Hnrnpc-B6J-VA
Gene Name
Product ID
S-CKO-02923
Gene Alias
D14Wsu171e; Hnrpc; Hnrpc1; Hnrpc2; hnRNPC1; hnRNPC2; hnrnp-C
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hnrnpcem1flox/Cya mice (Catalog S-CKO-02923) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111610
NCBI RefSeq
NM_016884
Target Region
Exon 4~5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Hnrnpc, also known as heterogeneous nuclear ribonucleoprotein C (C1/C2), is an essential and ubiquitously abundant protein involved in mRNA processing. It belongs to the heterogeneous nuclear ribonucleoproteins (hnRNPs) family of RNA-binding proteins (RBPs) which are crucial for nucleic acid metabolism. Hnrnpc is associated with various biological processes, and its dysregulation has been linked to multiple diseases [2].
In hepatocellular carcinoma (HCC), Hnrnpc down-regulation inhibits IL-6/STAT3-mediated metastasis by decreasing HIF1A expression, as it destabilizes HIF1A mRNA. Patients with positive Hnrnpc expression have decreased overall survival and increased recurrence rate [1].
In cervical cancer, knocking down Hnrnpc inhibits migration and invasion of cell lines, as Hnrnpc promotes lymphatic metastasis through m6A-dependent alternative splicing of FOXM1 [3].
In pancreatic cancer, overexpression of Hnrnpc in vitro increases cell proliferation, migration, invasion, and metastasis, and it promotes tumorigenesis in vivo by stabilizing IQGAP3 [4].
In conclusion, Hnrnpc plays a significant role in mRNA processing and is involved in various biological processes. Through gene knockout or knockdown studies in different cancer types such as HCC, cervical cancer, and pancreatic cancer, it has been shown to be closely related to tumor metastasis, proliferation, and patient prognosis. These findings highlight its potential as a biomarker and therapeutic target in cancer treatment.
References：
1. Liu, Danfei, Luo, Xiangyuan, Xie, Meng, Huang, Wenjie, Xia, Limin. 2022. HNRNPC downregulation inhibits IL-6/STAT3-mediated HCC metastasis by decreasing HIF1A expression. In Cancer science, 113, 3347-3361. doi:10.1111/cas.15494. https://pubmed.ncbi.nlm.nih.gov/35848884/
2. Niggl, Eva, Bouman, Arjan, Briere, Lauren C, Elgersma, Ype, van Esbroeck, Annelot C M. . HNRNPC haploinsufficiency affects alternative splicing of intellectual disability-associated genes and causes a neurodevelopmental disorder. In American journal of human genetics, 110, 1414-1435. doi:10.1016/j.ajhg.2023.07.005. https://pubmed.ncbi.nlm.nih.gov/37541189/
3. Liu, Yun-Yun, Xia, Meng, Chen, Zhi-Bo, Lu, Huai-Wu, Yao, Shu-Zhong. 2024. HNRNPC mediates lymphatic metastasis of cervical cancer through m6A-dependent alternative splicing of FOXM1. In Cell death & disease, 15, 732. doi:10.1038/s41419-024-07108-4. https://pubmed.ncbi.nlm.nih.gov/39375330/
4. Yang, Nannan, Liu, Lin, Liu, Xiaoyu, Lu, Jian, Wang, Zhongmin. 2022. hnRNPC Promotes Malignancy in Pancreatic Cancer through Stabilization of IQGAP3. In BioMed research international, 2022, 6319685. doi:10.1155/2022/6319685. https://pubmed.ncbi.nlm.nih.gov/35355828/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen