C57BL/6JCya-Hpdem1flox/Cya
Common Name
Hpd-flox
Product ID
S-CKO-02963
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-15445-Hpd-B6J-VA
Status
When using this mouse strain in a publication, please cite “Hpd-flox Mouse (Catalog S-CKO-02963) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Hpd-flox
Strain ID
CKOCMP-15445-Hpd-B6J-VA
Gene Name
Product ID
S-CKO-02963
Gene Alias
Fla, Flp, Laf, Hppd, 4HPPD
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031398
NCBI RefSeq
NM_008277
Target Region
Exon 7~9
Size of Effective Region
~2.8 kb
Overview of Gene Research
Hpd, also known as 4-hydroxyphenylpyruvate dioxygenase, is a critical enzyme in tyrosine metabolism [1,2]. Tyrosine metabolism pathways are essential for various biological processes, and Hpd's function is crucial in maintaining normal physiological states. Deficiency in Hpd can lead to tyrosinemia, highlighting its importance in this metabolic context [1,2].
In Ttc36-/-mice, which lack the molecular chaperone TTC36, Hpd expression in the liver is reduced. This reduction is due to enhanced STK33-mediated HPD T382 phosphorylation, leading to increased PELI1-mediated Hpd polyubiquitylation and degradation. These Ttc36-/-mice exhibit tyrosinemia, damage to hippocampal neurons, and learning and memory deficits, revealing the role of Hpd in preventing tyrosinemia-associated neurological disorders [2].
In breast cancer, Hpd protein expression is increased compared to adjacent normal tissues. High Hpd expression is positively correlated with histological grade and clinical stage, and inversely correlated with 10-year overall survival rates, suggesting Hpd may be a prognostic predictor and potential therapeutic target [1].
In conclusion, Hpd is vital for tyrosine metabolism and its dysregulation can lead to tyrosinemia and associated neurological damage as shown in gene-knockout-like mouse models. In addition, its abnormal expression in breast cancer is related to prognosis, emphasizing its significance in both metabolic-related diseases and cancer research.
References：
1. Wang, Xuanyu, Shang, Yongjun, Yang, Lina, Shan, Changliang, Liu, Shuangping. 2019. HPD overexpression predicts poor prognosis in breast cancer. In Pathology, research and practice, 215, 152524. doi:10.1016/j.prp.2019.152524. https://pubmed.ncbi.nlm.nih.gov/31277952/
2. Xie, Yajun, Lv, Xiaoyan, Ni, Dongsheng, Lu, Zhimin, Zhou, Qin. 2019. HPD degradation regulated by the TTC36-STK33-PELI1 signaling axis induces tyrosinemia and neurological damage. In Nature communications, 10, 4266. doi:10.1038/s41467-019-12011-0. https://pubmed.ncbi.nlm.nih.gov/31537781/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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