C57BL/6JCya-Prmt1em1flox/Cya
Common Name:
Prmt1-flox
Product ID:
S-CKO-02977
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Prmt1-flox
Strain ID
CKOCMP-15469-Prmt1-B6J-VA
Gene Name
Product ID
S-CKO-02977
Gene Alias
6720434D09Rik; Hrmt1l2; Mrmt1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prmt1em1flox/Cya mice (Catalog S-CKO-02977) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107843
NCBI RefSeq
NM_019830
Target Region
Exon 4~5
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
PRMT1, protein arginine methyltransferase 1, is the major protein arginine methyltransferase in mammals. It catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins, playing essential roles in numerous biological processes. Initially recognized for regulating chromatin dynamics via histone H4 arginine 3 (H4R3) methylation, it is now known to be involved in transcriptional regulation, signal transduction, DNA repair, and more [1].
In pancreatic cancer, deletion of PRMT1 in a KRAS-dependent mouse model delays cancer development. Multi-omics analyses show that PRMT1 depletion leads to global changes in chromatin accessibility and transcription, reducing glycolysis and tumorigenic capacity [3]. In hepatocellular carcinoma, FBXO7, an E3 ubiquitin ligase, binds PRMT1, induces its ubiquitination, and promotes proteosomal degradation, thus inhibiting serine synthesis and tumor growth [2]. In glioma, diminished PRMT1 expression in IDH1R132H gliomas is associated with reduced PTX3 levels, affecting ferritinophagy [4]. In colorectal cancer, PRMT1-mediated H4R3me2a recruits SMARCA4 to promote cancer progression by enhancing EGFR signaling, and knockdown or inhibition of PRMT1 attenuates tumor growth in a CRC mouse model [5].
In conclusion, PRMT1 is crucial for various biological functions including chromatin regulation, cell metabolism, and signal transduction. Studies using gene knockout and conditional knockout mouse models have revealed its significant roles in cancer development, such as in pancreatic, hepatocellular, glioma, and colorectal cancers, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Thiebaut, Charlène, Eve, Louisane, Poulard, Coralie, Le Romancer, Muriel. 2021. Structure, Activity, and Function of PRMT1. In Life (Basel, Switzerland), 11, . doi:10.3390/life11111147. https://pubmed.ncbi.nlm.nih.gov/34833023/
2. Luo, Li, Wu, Xingyun, Fan, Jiawu, Jiang, Jingwen, Wang, Kui. 2024. FBXO7 ubiquitinates PRMT1 to suppress serine synthesis and tumor growth in hepatocellular carcinoma. In Nature communications, 15, 4790. doi:10.1038/s41467-024-49087-2. https://pubmed.ncbi.nlm.nih.gov/38839752/
3. Ku, Bomin, Eisenbarth, David, Baek, Seonguk, Kong, Sun-Young, Lim, Dae-Sik. 2024. PRMT1 promotes pancreatic cancer development and resistance to chemotherapy. In Cell reports. Medicine, 5, 101461. doi:10.1016/j.xcrm.2024.101461. https://pubmed.ncbi.nlm.nih.gov/38460517/
4. Lathoria, Kirti, Gowda, Pruthvi, Umdor, Sonia B, Suri, Vaishali, Sen, Ellora. 2023. PRMT1 driven PTX3 regulates ferritinophagy in glioma. In Autophagy, 19, 1997-2014. doi:10.1080/15548627.2023.2165757. https://pubmed.ncbi.nlm.nih.gov/36647288/
5. Yao, Bing, Gui, Tao, Zeng, Xiangwei, Ju, Junyi, Zhao, Quan. 2021. PRMT1-mediated H4R3me2a recruits SMARCA4 to promote colorectal cancer progression by enhancing EGFR signaling. In Genome medicine, 13, 58. doi:10.1186/s13073-021-00871-5. https://pubmed.ncbi.nlm.nih.gov/33853662/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen