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C57BL/6JCya-Hsd17b4em1flox/Cya
Common Name:
Hsd17b4-flox
Product ID:
S-CKO-02985
Background:
C57BL/6JCya
Product Type
Age
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Basic Information
Strain Name
Hsd17b4-flox
Strain ID
CKOCMP-15488-Hsd17b4-B6J-VA
Gene Name
Hsd17b4
Product ID
S-CKO-02985
Gene Alias
17-beta-HSD; 17[b]-HSD; DBP; MFE-2; MFP2; MPF-2; Mfp-2; perMFE-2
Background
C57BL/6JCya
NCBI ID
15488
Modification
Conditional knockout
Chromosome
18
Phenotype
MGI:105089
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hsd17b4em1flox/Cya mice (Catalog S-CKO-02985) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025385
NCBI RefSeq
NM_008292
Target Region
Exon 8
Size of Effective Region
~1.1 kb
Detailed Document
Click here to download >>
Overview of Gene Research
HSD17B4, encoding 17β-hydroxysteroid dehydrogenase type 4, is a peroxisomal bifunctional enzyme pivotal for peroxisomal β-oxidation of very long-chain fatty acids (VLCFA) and acetyl-CoA synthesis [1,2,4]. It also plays a role in estradiol metabolism, with the highest levels of its mRNA transcription and specific activity found in the liver, kidney, ovary, and testes [4]. Mutations in HSD17B4 are associated with a fatal form of Zellweger syndrome [4].

In Hsd17B4 -knockout mice, acetate administration restores motor function, enhances primary cilia formation, and preserves the Purkinje layer, suggesting HSD17B4 deficiency impairs primary ciliogenesis and cilia-mediated signaling, and this can be rescued by acetyl-CoA [1]. In BT-474 breast cancer cells, HSD17B4 knockout suppresses cellular proliferation, enhances sensitivity to lapatinib, and leads to metabolic changes including VLCFA accumulation and PUFA decrease [2]. In prostate cancer cells, knockdown of HSD17B4 significantly suppresses proliferation, migration, and invasion, while overexpressing it has opposite effects [3].

In conclusion, HSD17B4 is crucial for peroxisomal metabolism, primary cilia regulation, and is involved in the progression of certain cancers. Gene-knockout mouse models have been instrumental in revealing its role in ciliopathy-like symptoms, breast cancer metabolism, and prostate cancer progression, providing insights into potential therapeutic targets for related diseases.

References:
1. Bae, Ji-Eun, Jang, Soyoung, Kim, Joon Bum, Ryoo, Zae Young, Cho, Dong-Hyung. 2025. HSD17B4 deficiency causes dysregulation of primary cilia and is alleviated by acetyl-CoA. In Nature communications, 16, 2663. doi:10.1038/s41467-025-57793-8. https://pubmed.ncbi.nlm.nih.gov/40102401/
2. Arai, Nobuaki, Hattori, Naoko, Yamashita, Satoshi, Mukai, Hirofumi, Ushijima, Toshikazu. 2023. HSD17B4 methylation enhances glucose dependence of BT-474 breast cancer cells and increases lapatinib sensitivity. In Breast cancer research and treatment, 201, 317-328. doi:10.1007/s10549-023-07013-y. https://pubmed.ncbi.nlm.nih.gov/37378696/
3. Huang, Huichao, Liu, Ruijie, Huang, Yahui, Zhang, Ye, Chen, Yongheng. 2020. Acetylation-mediated degradation of HSD17B4 regulates the progression of prostate cancer. In Aging, 12, 14699-14717. doi:10.18632/aging.103530. https://pubmed.ncbi.nlm.nih.gov/32678070/
4. de Launoit, Y, Adamski, J. . Unique multifunctional HSD17B4 gene product: 17beta-hydroxysteroid dehydrogenase 4 and D-3-hydroxyacyl-coenzyme A dehydrogenase/hydratase involved in Zellweger syndrome. In Journal of molecular endocrinology, 22, 227-40. doi:. https://pubmed.ncbi.nlm.nih.gov/10343282/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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