C57BL/6JCya-Hsd17b7em1flox/Cya
Common Name:
Hsd17b7-flox
Product ID:
S-CKO-02986
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hsd17b7-flox
Strain ID
CKOCMP-15490-Hsd17b7-B6J-VA
Gene Name
Product ID
S-CKO-02986
Gene Alias
ERG27
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hsd17b7em1flox/Cya mice (Catalog S-CKO-02986) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027989
NCBI RefSeq
NM_010476
Target Region
Exon 3~4
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Hsd17b7, also known as 17β-hydroxysteroid dehydrogenase type 7 or prolactin receptor-associated protein, is an enzyme-encoding gene. It has a dual function in sex steroid and cholesterol biosynthesis [1,3,4,6]. In sex steroid synthesis, it converts estrone to estradiol, and in cholesterol biosynthesis, it acts as the 3-ketoreductase in the postsqualene cholesterol biosynthesis pathway [3,5]. This gene is of great biological importance as it is involved in multiple physiological processes, and genetic models can be used to further explore its functions.
In a knockout mouse model, deletion of exons 1-4 of the Hsd17b7 gene led to no viable null mice. Hsd17b7 null embryos were alive on d 8.5 and d 9.5 but by d 10.5, they suffered from severe brain malformation and heart defect, likely due to the role of this enzyme in cholesterol synthesis required for brain development [6]. Also, genetic suppression of Hsd17B7 increased the formation of oligodendrocytes, suggesting its role in neurodegenerative-related contexts [2].
In conclusion, Hsd17b7 is crucial for embryonic development and fetal survival due to its role in cholesterol biosynthesis [6]. Its function in promoting oligodendrocyte formation indicates its potential significance in neurodegenerative diseases [2]. Studies using knockout mouse models have provided valuable insights into the essential biological functions of Hsd17b7 and its implications in specific disease areas.
References:
1. Xu, Xiaoying, Tassone, Beatrice, Ostano, Paola, Lefort, Karine, Dotto, Gian Paolo. 2021. HSD17B7 gene in self-renewal and oncogenicity of keratinocytes from Black versus White populations. In EMBO molecular medicine, 13, e14133. doi:10.15252/emmm.202114133. https://pubmed.ncbi.nlm.nih.gov/34185380/
2. Pleshinger, Matthew J, Friedrich, Ryan M, Hubler, Zita, Tesar, Paul J, Adams, Drew J. 2021. Inhibition of SC4MOL and HSD17B7 shifts cellular sterol composition and promotes oligodendrocyte formation. In RSC chemical biology, 3, 56-68. doi:10.1039/d1cb00145k. https://pubmed.ncbi.nlm.nih.gov/35128409/
3. Seth, Gargi, McIvor, R Scott, Hu, Wei-Shou. 2005. 17Beta-hydroxysteroid dehydrogenase type 7 (Hsd17b7) reverts cholesterol auxotrophy in NS0 cells. In Journal of biotechnology, 121, 241-52. doi:. https://pubmed.ncbi.nlm.nih.gov/16126295/
4. Shehu, Aurora, Albarracin, Constance, Devi, Y Sangeeta, Frasor, Jonna, Gibori, Geula. 2011. The stimulation of HSD17B7 expression by estradiol provides a powerful feed-forward mechanism for estradiol biosynthesis in breast cancer cells. In Molecular endocrinology (Baltimore, Md.), 25, 754-66. doi:10.1210/me.2010-0261. https://pubmed.ncbi.nlm.nih.gov/21372145/
5. Ohnesorg, Thomas, Adamski, Jerzy. 2005. Analysis of the 5' flanking regions of human and murine HSD17B7: identification of a cholesterol dependent enhancer region. In Molecular and cellular endocrinology, 248, 164-7. doi:. https://pubmed.ncbi.nlm.nih.gov/16356630/
6. Shehu, Aurora, Mao, Jifang, Gibori, Gil B, Kiyokawa, Hiroaki, Gibori, Geula. 2008. Prolactin receptor-associated protein/17beta-hydroxysteroid dehydrogenase type 7 gene (Hsd17b7) plays a crucial role in embryonic development and fetal survival. In Molecular endocrinology (Baltimore, Md.), 22, 2268-77. doi:10.1210/me.2008-0165. https://pubmed.ncbi.nlm.nih.gov/18669642/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen