C57BL/6JCya-Hspd1em1flox/Cya
Common Name:
Hspd1-flox
Product ID:
S-CKO-02993
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hspd1-flox
Strain ID
CKOCMP-15510-Hspd1-B6J-VA
Gene Name
Product ID
S-CKO-02993
Gene Alias
60kDa; CPN60; HSP-60; HSP-65; Hsp60
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hspd1em1flox/Cya mice (Catalog S-CKO-02993) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027123
NCBI RefSeq
NM_010477
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Hspd1, also known as mitochondrial heat shock protein 60 (HSP60), is a mitochondrial chaperone that functions as folding machinery for mitochondrial proteins imported into the mitochondrial matrix space [2]. It has been implicated in multiple biological processes and associated with various diseases, highlighting its overall biological importance. Genetic models could potentially be valuable for further studying its functions.
In osteosarcoma, Hspd1 expression is increased and is associated with a negative prognosis. It promotes the regulation of proliferation, metastasis, and apoptosis in osteosarcoma by facilitating the epithelial-mesenchymal transition (EMT) and activating the AKT/mTOR signaling pathway. Mechanistically, it interacts with ATP5A1 to reduce its K48-linked ubiquitination and degradation, ultimately activating the AKT/mTOR pathway [1].
In oral squamous cell carcinoma, Hspd1 represses E-cadherin expression, promoting cell invasion and migration, and is associated with poor prognosis [3].
In contrast, in pituitary adenomas, the expression of Hspd1 is significantly lower in invasive ones, and its down-regulation may be related to the invasion process, mitochondria-related pathways, and immune cell regulation, serving as a predictive indicator for identifying invasive pituitary adenomas [4].
In conclusion, Hspd1 plays a crucial role in protein folding within mitochondria. Its dysregulation is associated with various diseases such as osteosarcoma, oral squamous cell carcinoma, and pituitary adenomas. The study of Hspd1 using genetic models, as hinted in the research, could further reveal its complex functions in these disease conditions, potentially providing new therapeutic targets.
References:
1. Zhang, Yiming, Pan, Ruilin, Li, Kun, He, Rongrong, Wang, Huajun. 2024. HSPD1 Supports Osteosarcoma Progression through Stabilizing ATP5A1 and thus Activation of AKT/mTOR Signaling. In International journal of biological sciences, 20, 5162-5190. doi:10.7150/ijbs.100015. https://pubmed.ncbi.nlm.nih.gov/39430254/
2. Cömert, Cagla, Brick, Lauren, Ang, Debbie, Fernandez-Guerra, Paula, Bross, Peter. 2020. A recurrent de novo HSPD1 variant is associated with hypomyelinating leukodystrophy. In Cold Spring Harbor molecular case studies, 6, . doi:10.1101/mcs.a004879. https://pubmed.ncbi.nlm.nih.gov/32532876/
3. Kang, Bor-Hwang, Shu, Chih-Wen, Chao, Jian-Kang, Ger, Luo-Ping, Liu, Pei-Feng. 2019. HSPD1 repressed E-cadherin expression to promote cell invasion and migration for poor prognosis in oral squamous cell carcinoma. In Scientific reports, 9, 8932. doi:10.1038/s41598-019-45489-1. https://pubmed.ncbi.nlm.nih.gov/31222140/
4. Zhang, Yu, Ma, Xin, Liu, Congyu, Liu, Pinan, Yang, Zhijun. 2024. Identification of HSPD1 as a novel invasive biomarker associated with mitophagy in pituitary adenomas. In Translational oncology, 41, 101886. doi:10.1016/j.tranon.2024.101886. https://pubmed.ncbi.nlm.nih.gov/38290248/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen