C57BL/6JCya-Htr2cem1flox/Cya
Common Name:
Htr2c-flox
Product ID:
S-CKO-03006
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Htr2c-flox
Strain ID
CKOCMP-15560-Htr2c-B6J-VA
Gene Name
Product ID
S-CKO-03006
Gene Alias
5-HT-1C; 5-HT-2C; 5-HT1C; 5-HT2C; 5-HT2cR; 5-HTR2C; 5HT1c; Htr1c; SR1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Htr2cem1flox/Cya mice (Catalog S-CKO-03006) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000036303
NCBI RefSeq
NM_008312
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Htr2c, the serotonin 2C receptor, is a class G protein-coupled receptor (GPCR). It is enriched in the hypothalamus and brain stem, regulating energy homeostasis, such as feeding and glucose metabolism. It has also been associated with neural activity regulation in the context of mood disorders [1,2].
Using mouse genetic models where Htr2c can be selectively deleted or restored, it was found that Htr2c in forebrain Sim1 neurons promotes food intake, counteracting the anorectic effect of serotonergic appetite suppressants. Activation of Htr2c in the paraventricular nucleus of the hypothalamus (PVH) inhibits PVH neurons through a Gαi/o -dependent mechanism [3].
In conclusion, Htr2c plays a crucial role in metabolic regulation, especially in feeding behavior. The gene knockout and restoration models in mice have provided insights into its function in the context of appetite regulation, which may contribute to understanding and treating metabolic disorders related to abnormal feeding [1,3].
References：
1. Yao, Ting, He, Jiehui, Cui, Zhicheng, Wang, Ru, Liu, Tiemin. 2021. Central 5-HTR2C in the Control of Metabolic Homeostasis. In Frontiers in endocrinology, 12, 694204. doi:10.3389/fendo.2021.694204. https://pubmed.ncbi.nlm.nih.gov/34367066/
2. Lu, Kaiqin, Hong, Yuan, Tao, Mengdan, Guo, Xing, Liu, Yan. 2022. Depressive patient-derived GABA interneurons reveal abnormal neural activity associated with HTR2C. In EMBO molecular medicine, 15, e16364. doi:10.15252/emmm.202216364. https://pubmed.ncbi.nlm.nih.gov/36373384/
3. Yoo, Eun-Seon, Li, Li, Jia, Lin, Sohn, Jong-Woo, Liu, Chen. . Gαi/o-coupled Htr2c in the paraventricular nucleus of the hypothalamus antagonizes the anorectic effect of serotonin agents. In Cell reports, 37, 109997. doi:10.1016/j.celrep.2021.109997. https://pubmed.ncbi.nlm.nih.gov/34788630/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen