C57BL/6JCya-Elavl3em1flox/Cya
Common Name:
Elavl3-flox
Product ID:
S-CKO-03016
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Elavl3-flox
Strain ID
CKOCMP-15571-Elavl3-B6J-VA
Gene Name
Product ID
S-CKO-03016
Gene Alias
2600009P04Rik; Huc; PLE21; mHuC
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Elavl3em1flox/Cya mice (Catalog S-CKO-03016) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003501
NCBI RefSeq
NM_010487
Target Region
Exon 4~5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Elavl3, also known as neuronal Elav-like 3 or HuC, is an RNA-binding protein. It belongs to the nElavl family and is highly expressed in the adult brain. Elavl3 regulates RNA stability, alternative splicing, and polyadenylation site choice, which are crucial for axonal and synaptic structures, as well as neuronal differentiation, maturation, and polarity regulation [2,3,5].
In Elavl3-/-mice, there are slowly progressive motor deficits leading to severe cerebellar ataxia, with swollen axons in Purkinje cells, disruption of synaptic formation, and deficits in axonal transport and neuronal polarity [2]. Also, in these mice, the alternative splicing of AnkyrinG exon 34 is misregulated, affecting neuronal polarity [3]. In neuroendocrine prostate cancer, ELAVL3 is specifically upregulated, forming a positive feedback loop with MYCN. Pharmacological inhibition of ELAVL3 suppresses tumor growth in mouse models [1]. In neuroblastoma, targeted silencing of ELAVL3 down-regulates MYCN gene expression and reduces malignancy [4].
In conclusion, Elavl3 is essential for maintaining axonal homeostasis, regulating neuronal polarity, and is involved in the development of certain cancers. Studies using Elavl3 knockout mouse models have provided valuable insights into its functions in normal neuronal processes and disease conditions such as neurodegenerative diseases and cancers [1,2,3,4].
References:
1. Ji, Yiyi, Zhang, Weiwei, Shen, Kai, Wang, Qi, Xue, Wei. 2023. The ELAVL3/MYCN positive feedback loop provides a therapeutic target for neuroendocrine prostate cancer. In Nature communications, 14, 7794. doi:10.1038/s41467-023-43676-3. https://pubmed.ncbi.nlm.nih.gov/38016952/
2. Ogawa, Yuki, Kakumoto, Kyoko, Yoshida, Tetsu, Okano, Hideyuki, Okano, Hirotaka James. 2018. Elavl3 is essential for the maintenance of Purkinje neuron axons. In Scientific reports, 8, 2722. doi:10.1038/s41598-018-21130-5. https://pubmed.ncbi.nlm.nih.gov/29426875/
3. Ogawa, Yuki, Yamaguchi, Junji, Yano, Masato, Uchiyama, Yasuo, Okano, Hirotaka James. 2018. Elavl3 regulates neuronal polarity through the alternative splicing of an embryo-specific exon in AnkyrinG. In Neuroscience research, 135, 13-20. doi:10.1016/j.neures.2018.03.008. https://pubmed.ncbi.nlm.nih.gov/29614249/
4. Hong, Peng, Hu, Zaihong, Lin, Jie, Lin, Tao, Wei, Guanghui. 2024. Multi-omics revealed that ELAVL3 regulates MYCN in neuroblastoma via immunogenic cell death: Risk stratification and experimental research. In International journal of biological macromolecules, 282, 137045. doi:10.1016/j.ijbiomac.2024.137045. https://pubmed.ncbi.nlm.nih.gov/39486730/
5. Grassi, Elena, Santoro, Roberto, Umbach, Alessandro, DiCunto, Ferdinando, Merlo, Giorgio R. 2019. Choice of Alternative Polyadenylation Sites, Mediated by the RNA-Binding Protein Elavl3, Plays a Role in Differentiation of Inhibitory Neuronal Progenitors. In Frontiers in cellular neuroscience, 12, 518. doi:10.3389/fncel.2018.00518. https://pubmed.ncbi.nlm.nih.gov/30687010/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen