C57BL/6JCya-Iappem1flox/Cya
Common Name:
Iapp-flox
Product ID:
S-CKO-03021
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Iapp-flox
Strain ID
CKOCMP-15874-Iapp-B6J-VA
Gene Name
Product ID
S-CKO-03021
Gene Alias
DAP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Iappem1flox/Cya mice (Catalog S-CKO-03021) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041993
NCBI RefSeq
NM_010491
Target Region
Exon 3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Iapp, also known as Islet Amyloid Polypeptide or amylin, is a 37-amino-acid pancreatic β-cell hormone. It activates specific multisubunit G protein-coupled receptors, and its major role is as a glucoregulatory hormone, being an important regulator of energy metabolism [2].
In type 2 diabetes, Iapp forms cytotoxic oligomers and amyloid fibrils in islets. These aggregates are toxic to β-cells, inducing β-cell death, dysfunction, and islet inflammation. In transgenic rodents overexpressing human Iapp (hIapp), intracellular pro-apoptotic signals can be generated, indicating that defects in intracellular handling of hIapp can induce cytotoxicity [1]. Also, in mice, overexpression of soluble rodent or oligomer-prone human Iapp induced changes in the islet transcriptome similar to those in prediabetes and type 2 diabetes in humans, including decreased expression of genes conferring β-cell identity and induction of islet inflammation [3].
In conclusion, Iapp is crucial for glucose metabolism and energy regulation. Studies using transgenic rodent models, especially those overexpressing hIapp, have revealed its role in β-cell cytotoxicity, islet inflammation, and the development of type 2 diabetes. Understanding Iapp's function provides insights into the pathophysiology of diabetes and potential therapeutic targets.
References:
1. Raleigh, Daniel, Zhang, Xiaoxue, Hastoy, Benoît, Clark, Anne. 2017. The β-cell assassin: IAPP cytotoxicity. In Journal of molecular endocrinology, 59, R121-R140. doi:10.1530/JME-17-0105. https://pubmed.ncbi.nlm.nih.gov/28811318/
2. Hay, Debbie L, Chen, Steve, Lutz, Thomas A, Parkes, David G, Roth, Jonathan D. . Amylin: Pharmacology, Physiology, and Clinical Potential. In Pharmacological reviews, 67, 564-600. doi:10.1124/pr.115.010629. https://pubmed.ncbi.nlm.nih.gov/26071095/
3. Blencowe, Montgomery, Furterer, Allison, Wang, Qing, Butler, Peter C, Gurlo, Tatyana. 2021. IAPP-induced beta cell stress recapitulates the islet transcriptome in type 2 diabetes. In Diabetologia, 65, 173-187. doi:10.1007/s00125-021-05569-2. https://pubmed.ncbi.nlm.nih.gov/34554282/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen