C57BL/6JCya-Irf8em1flox/Cya
Common Name:
Irf8-flox
Product ID:
S-CKO-03026
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Irf8-flox
Strain ID
CKOCMP-15900-Irf8-B6J-VA
Gene Name
Product ID
S-CKO-03026
Gene Alias
ICSBP; IRF-8; Icsbp1; Myls
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Irf8em1flox/Cya mice (Catalog S-CKO-03026) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047737
NCBI RefSeq
NM_008320
Target Region
Exon 5
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Irf8, also known as interferon regulatory factor 8, is a transcription factor of the IRF protein family. It is essential for myeloid cell lineage commitment and differentiation, and is involved in pathways like antigen processing and presentation, cytokine and chemokine expression, and response to various stimuli. It plays a crucial role in the immune system, influencing the development and function of immune cells [2,4]. Genetic models, such as gene knockout (KO) mouse models, have been valuable in studying its functions.
In KO mouse models, deletion of Irf8 leads to massive accumulation of CD11b+Gr1+ immature myeloid cells, particularly polymorphonuclear myeloid-derived suppressor cell-like cells, which can promote tumor immune evasion [2]. In breast cancer murine models, TAM-specific Irf8 deletion prevented exhaustion of cancer-cell-reactive CTLs and suppressed tumor growth, indicating that Irf8 in tumor-associated macrophages promotes T cell exhaustion [1]. In microglia, constitutive and postnatal Irf8 deletion led to a loss of microglia identity, gain of disease-associated microglia-like genes, and reduced interaction with amyloidβ plaques in the 5xFAD model [3].
In conclusion, Irf8 is vital for the development and function of immune cells and plays a role in multiple biological processes. Studies using Irf8 KO/CKO mouse models have revealed its significance in cancer, where it can impact tumor growth through T cell exhaustion, and in microglia-related diseases like those associated with amyloidβ plaques. These findings enhance our understanding of disease mechanisms and may offer potential therapeutic targets.
References:
1. Nixon, Briana G, Kuo, Fengshen, Ji, LiangLiang, Hakimi, A Ari, Li, Ming O. 2022. Tumor-associated macrophages expressing the transcription factor IRF8 promote T cell exhaustion in cancer. In Immunity, 55, 2044-2058.e5. doi:10.1016/j.immuni.2022.10.002. https://pubmed.ncbi.nlm.nih.gov/36288724/
2. Moorman, Hannah R, Reategui, Yazmin, Poschel, Dakota B, Liu, Kebin. 2022. IRF8: Mechanism of Action and Health Implications. In Cells, 11, . doi:10.3390/cells11172630. https://pubmed.ncbi.nlm.nih.gov/36078039/
3. Saeki, Keita, Pan, Richard, Lee, Eunju, Kurotaki, Daisuke, Ozato, Keiko. 2024. IRF8 defines the epigenetic landscape in postnatal microglia, thereby directing their transcriptome programs. In Nature immunology, 25, 1928-1942. doi:10.1038/s41590-024-01962-2. https://pubmed.ncbi.nlm.nih.gov/39313544/
4. Salem, Sandra, Salem, David, Gros, Philippe. 2020. Role of IRF8 in immune cells functions, protection against infections, and susceptibility to inflammatory diseases. In Human genetics, 139, 707-721. doi:10.1007/s00439-020-02154-2. https://pubmed.ncbi.nlm.nih.gov/32232558/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen