C57BL/6JCya-Idh1em1flox/Cya
Common Name:
Idh1-flox
Product ID:
S-CKO-03032
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Idh1-flox
Strain ID
CKOCMP-15926-Idh1-B6J-VA
Gene Name
Product ID
S-CKO-03032
Gene Alias
E030024J03Rik; Id-1; Idh-1; Idpc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Idh1em1flox/Cya mice (Catalog S-CKO-03032) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169032
NCBI RefSeq
NM_001111320
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Idh1, or Isocitrate dehydrogenase 1, is a key metabolic enzyme in the tricarboxylic acid (TCA) cycle. It catalyzes the conversion of isocitrate to α -ketoglutarate (α -KG) and generates NADPH, playing a crucial role in cellular respiration. Changes in its expression levels or gene mutations can affect dioxygenase activity and the body's detoxification mechanism [4].
Mutations in Idh1 have been observed in several tumors, such as glioma, acute myeloid leukemia, and chondrosarcoma. Mutant Idh1 increases the conversion of α -KG to 2 -hydroxyglutarate (2 -HG), leading to epigenetic dysregulation, altered gene expression, and impaired cell differentiation. Small-molecule inhibitors targeting mutant Idh1 (mIdh1) are emerging as a therapeutic approach, like the approved inhibitor AG-120 (ivosidenib) for treating Idh1-mutated AML [1,3]. In hepatocellular carcinoma, scutellarin can activate Idh1, limit glycolysis, and activate the tumor immune microenvironment [2].
In conclusion, Idh1 is essential for cellular metabolism, especially in the TCA cycle. Research on Idh1, particularly its mutant forms, has significant implications for understanding and treating various cancers. The development of Idh1-targeted inhibitors offers new hope for cancer patients, highlighting the importance of studying this gene in the context of disease-related biological processes.
References:
1. Tian, Wangqi, Zhang, Weitong, Wang, Yifan, Tang, Yuping, Yao, Xiaojun. 2022. Recent advances of IDH1 mutant inhibitor in cancer therapy. In Frontiers in pharmacology, 13, 982424. doi:10.3389/fphar.2022.982424. https://pubmed.ncbi.nlm.nih.gov/36091829/
2. Cui, Zhao, Li, Caifeng, Liu, Wei, Yang, Hongjun, Chen, Peng. 2024. Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progression. In Cell death & disease, 15, 267. doi:10.1038/s41419-024-06625-6. https://pubmed.ncbi.nlm.nih.gov/38622131/
3. Megías-Vericat, Juan Eduardo, Ballesta-López, Octavio, Barragán, Eva, Montesinos, Pau. 2019. IDH1-mutated relapsed or refractory AML: current challenges and future prospects. In Blood and lymphatic cancer : targets and therapy, 9, 19-32. doi:10.2147/BLCTT.S177913. https://pubmed.ncbi.nlm.nih.gov/31413655/
4. Ni, Yingqian, Shen, Peibo, Wang, Xingchen, Luo, Huiyuan, Han, Xiuzhen. 2023. The roles of IDH1 in tumor metabolism and immunity. In Future oncology (London, England), 18, 3941-3953. doi:10.2217/fon-2022-0583. https://pubmed.ncbi.nlm.nih.gov/36621781/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen