C57BL/6JCya-Cxcl10em1flox/Cya
Common Name:
Cxcl10-flox
Product ID:
S-CKO-03039
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cxcl10-flox
Strain ID
CKOCMP-15945-Cxcl10-B6J-VA
Gene Name
Product ID
S-CKO-03039
Gene Alias
C7; CRG-2; INP10; IP-10; IP10; Ifi10; Scyb10; gIP-10; mob-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cxcl10em1flox/Cya mice (Catalog S-CKO-03039) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038816
NCBI RefSeq
NM_021274
Target Region
Exon 2~3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
CXCL10, also known as chemokine (C-X-C motif) ligand 10, is a 10 kDa protein categorized as a Th1-chemokine. It binds to the receptor CXCR3, regulating immune responses by activating and recruiting leukocytes such as T cells, eosinophils, and monocytes [4]. The CXCL10-CXCR3 signaling is involved in multiple biological processes like T cell tumor infiltration, pain regulation, and inflammatory responses [1,2].
In a mouse model of vitiligo, mice lacking Cxcl10 or treated with CXCL10-neutralizing antibody developed minimal depigmentation, indicating CXCL10 is critical for the progression and maintenance of vitiligo [3]. In mice with spinal nerve ligation, intra-DRG injection of Cxcr3 shRNA attenuated mechanical allodynia and heat hyperalgesia, suggesting that CXCL10-activated CXCR3 mediates p38 and ERK activation in DRG neurons, enhancing neuronal excitability and contributing to neuropathic pain [2].
In conclusion, CXCL10 plays a significant role in immune-related biological processes and disease conditions. Findings from gene-knockout or neutralization experiments in mouse models have revealed its importance in diseases such as vitiligo and neuropathic pain, highlighting its potential as a therapeutic target in these areas.
References:
1. Li, Xiaofei, Lu, Mingjie, Yuan, Manman, Wu, Xingxin, Gu, Yanhong. 2022. CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy. In Oncoimmunology, 11, 2118210. doi:10.1080/2162402X.2022.2118210. https://pubmed.ncbi.nlm.nih.gov/36092638/
2. Kong, Yan-Fang, Sha, Wei-Lin, Wu, Xiao-Bo, Ma, Ling-Jie, Gao, Yong-Jing. 2020. CXCL10/CXCR3 Signaling in the DRG Exacerbates Neuropathic Pain in Mice. In Neuroscience bulletin, 37, 339-352. doi:10.1007/s12264-020-00608-1. https://pubmed.ncbi.nlm.nih.gov/33196963/
3. Rashighi, Mehdi, Agarwal, Priti, Richmond, Jillian M, Luster, Andrew D, Harris, John E. . CXCL10 is critical for the progression and maintenance of depigmentation in a mouse model of vitiligo. In Science translational medicine, 6, 223ra23. doi:10.1126/scitranslmed.3007811. https://pubmed.ncbi.nlm.nih.gov/24523323/
4. Lee, Eun Young, Lee, Zang-Hee, Song, Yeong Wook. 2008. CXCL10 and autoimmune diseases. In Autoimmunity reviews, 8, 379-83. doi:10.1016/j.autrev.2008.12.002. https://pubmed.ncbi.nlm.nih.gov/19105984/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen