C57BL/6JCya-Ifit2em1flox/Cya
Common Name:
Ifit2-flox
Product ID:
S-CKO-03042
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ifit2-flox
Strain ID
CKOCMP-15958-Ifit2-B6J-VA
Gene Name
Product ID
S-CKO-03042
Gene Alias
GARG-39; IFI-54K; Ifi54; P54
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ifit2em1flox/Cya mice (Catalog S-CKO-03042) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102826
NCBI RefSeq
NM_008332
Target Region
Exon 3
Size of Effective Region
~4.2 kb
Detailed Document
Overview of Gene Research
Ifit2, also known as IFN-stimulated gene 54 (ISG54), is a member of the interferon-stimulated gene (ISG) family. It plays crucial roles in antiviral and antitumor activities, and is involved in pathways such as immune responses, cell adhesion, and oxidative stress [1,3,7]. Genetic models, especially knockout mouse models, are valuable for studying its functions.
In Ifit2-deficient mice infected with murine-β -coronavirus RSA59, there is extensive viral spread throughout the spinal cord gray and white matter, along with impaired microglial activation, T cell infiltration, and severe chronic demyelination, highlighting Ifit2's role in promoting acute neuroinflammation and limiting chronic demyelination [4]. In oral cancer, IFIT2 depletion promotes cancer stem-cell-like phenotypes, and in oral squamous cell carcinoma, IFIT2-depleted metastatic cells induce muscle atrophy and cancer cachexia in mice [2,5]. In esophageal cancer, decreased IFIT2 expression increases cell viability, invasion, and migration, and is related to epithelial-to-mesenchymal transition [6]. In renal cancer, higher IFIT2 expression is associated with better overall survival [7]. In osteosarcoma, L3MBTL2 acts as a tumor suppressor by transcriptionally repressing IFIT2 [8].
In conclusion, Ifit2 is essential in antiviral defense and tumor-related processes. Studies using Ifit2 knockout mouse models have significantly advanced our understanding of its role in neurological diseases like viral-induced demyelination, as well as in various cancers, including oral, esophageal, renal, and osteosarcoma.
References:
1. Xu, Qiong-Cong, Tien, Yi-Chih, Shi, Yin-Hao, Zhao, Wei, Yin, Xiao-Yu. 2022. METTL3 promotes intrahepatic cholangiocarcinoma progression by regulating IFIT2 expression in an m6A-YTHDF2-dependent manner. In Oncogene, 41, 1622-1633. doi:10.1038/s41388-022-02185-1. https://pubmed.ncbi.nlm.nih.gov/35094011/
2. Lai, Kuo-Chu, Regmi, Prabha, Liu, Chung-Ji, Lo, Jeng-Fan, Lee, Te-Chang. 2023. IFIT2 Depletion Promotes Cancer Stem Cell-like Phenotypes in Oral Cancer. In Biomedicines, 11, . doi:10.3390/biomedicines11030896. https://pubmed.ncbi.nlm.nih.gov/36979874/
3. Chen, Haoqiang, Wu, Haiyan, Wang, Qian, Zhang, Hong. 2024. IFIT2 mediates iron retention and cholesterol efflux in atherosclerosis. In International immunopharmacology, 142, 113131. doi:10.1016/j.intimp.2024.113131. https://pubmed.ncbi.nlm.nih.gov/39276454/
4. Sharma, Madhav, Chakravarty, Debanjana, Hussain, Afaq, Sen, Ganes C, Das Sarma, Jayasri. 2023. Ifit2 restricts murine coronavirus spread to the spinal cord white matter and its associated myelin pathology. In Journal of virology, 97, e0074923. doi:10.1128/jvi.00749-23. https://pubmed.ncbi.nlm.nih.gov/37504572/
5. Lai, Kuo-Chu, Hong, Zi-Xuan, Hsieh, Jyh-Gang, Yang, Cheng-Han, Chen, Yan-Ru. 2022. IFIT2-depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice. In Journal of cachexia, sarcopenia and muscle, 13, 1314-1328. doi:10.1002/jcsm.12943. https://pubmed.ncbi.nlm.nih.gov/35170238/
6. Chen, J, Liu, Y, Zhu, Y, Chen, L, Jiang, J. 2022. STAT1/IFIT2 signaling pathway is involved in PD-L1-mediated epithelial-to-mesenchymal transition in human esophageal cancer. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 24, 927-940. doi:10.1007/s12094-021-02743-1. https://pubmed.ncbi.nlm.nih.gov/35107757/
7. Xu, Bin, Zhu, Yu-Lan, Fan, Jia-Lin, Chen, Lu-Jun, Jiang, Jing-Ting. . Clinical significance of IFIT2 expression in human renal cancer tissues. In Translational cancer research, 9, 3214-3221. doi:10.21037/tcr.2020.04.10. https://pubmed.ncbi.nlm.nih.gov/35117687/
8. Zhong, Li, Wang, Jingxuan, Chen, Wanqi, Kang, Tiebang, Liao, Dan. 2023. Augmenting L3MBTL2-induced condensates suppresses tumor growth in osteosarcoma. In Science advances, 9, eadi0889. doi:10.1126/sciadv.adi0889. https://pubmed.ncbi.nlm.nih.gov/37992172/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen