C57BL/6JCya-Ifngem1flox/Cya
Common Name:
Ifng-flox
Product ID:
S-CKO-03051
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ifng-flox
Strain ID
CKOCMP-15978-Ifng-B6J-VA
Gene Name
Product ID
S-CKO-03051
Gene Alias
IFN-g; If2f; Ifg
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ifngem1flox/Cya mice (Catalog S-CKO-03051) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000068592
NCBI RefSeq
NM_008337
Target Region
Exon 2~3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Ifng, encoding Interferon gamma, is a key cytokine in the immune system. It plays a crucial role in enhancing immune function, such as promoting the activation of immune cells. It is involved in multiple pathways, including those related to immune responses against pathogens and in cancer immunology. Its dysregulation is associated with various diseases, highlighting its biological importance. Genetic models, like gene knockout (KO) or conditional knockout (CKO) mouse models, can be valuable for studying Ifng's functions [2-10].
In autism spectrum disorder (ASD) children, there is a significant up-regulation of Ifng compared to controls, indicating a disruption in the IFNG/IFNG-AS1 regulatory network which may contribute to ASD's chronic inflammatory aspect [1]. In colon adenocarcinoma (COAD), Ifng is significantly correlated with tumor immunotherapy biomarkers, and high Ifng expression is related to longer disease-free survival. Also, autophagy and Ifng are negatively related to cisplatin chemosensitivity [2]. Regarding tuberculosis, Ifng gene polymorphisms show associations with the disease in the Tibetan population, with certain alleles acting as protective or risk factors [3,4]. In atopic dermatitis, high and low Ifng-expressing subgroups have distinct features, corresponding to intrinsic and extrinsic AD classifications [5]. Cholesterol can promote Ifng mRNA expression in CD4+ effector/memory cells via SGK1 activation [6].
In summary, Ifng is essential for immune function and is implicated in multiple disease conditions including ASD, COAD, tuberculosis, and atopic dermatitis. Model-based research, such as through KO/CKO mouse models, can further reveal its functions in these diseases, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Fallah, Hamid, Sayad, Arezou, Ranjbaran, Fatemeh, Ghafouri-Fard, Soudeh, Taheri, Mohammad. 2019. IFNG/IFNG-AS1 expression level balance: implications for autism spectrum disorder. In Metabolic brain disease, 35, 327-333. doi:10.1007/s11011-019-00510-4. https://pubmed.ncbi.nlm.nih.gov/31728886/
2. Yue, Taohua, Cai, Yunlong, Zhu, Jing, Wang, Pengyuan, Rong, Long. 2023. Autophagy-related IFNG is a prognostic and immunochemotherapeutic biomarker of COAD patients. In Frontiers in immunology, 14, 1064704. doi:10.3389/fimmu.2023.1064704. https://pubmed.ncbi.nlm.nih.gov/36756126/
3. Wu, S-Q, Ding, X-J, Yang, Q-L, Wang, M-G, He, J-Q. . IFNG and IFNGR1 polymorphisms are associated with tuberculosis: a case-control study. In European review for medical and pharmacological sciences, 26, 8777-8787. doi:10.26355/eurrev_202212_30549. https://pubmed.ncbi.nlm.nih.gov/36524496/
4. Wu, Shouquan, Wang, Yu, Zhang, Miaomiao, Wang, Minggui, He, Jian-Qing. 2019. Genetic variants in IFNG and IFNGR1 and tuberculosis susceptibility. In Cytokine, 123, 154775. doi:10.1016/j.cyto.2019.154775. https://pubmed.ncbi.nlm.nih.gov/31310896/
5. Wasserer, Sophia, Jargosch, Manja, Mayer, Kristine E, Eyerich, Kilian, Lauffer, Felix. 2024. Characterization of High and Low IFNG-Expressing Subgroups in Atopic Dermatitis. In International journal of molecular sciences, 25, . doi:10.3390/ijms25116158. https://pubmed.ncbi.nlm.nih.gov/38892346/
6. Hanin, Aurélie, Comi, Michela, Sumida, Tomokazu S, Hafler, David A. 2024. Cholesterol promotes IFNG mRNA expression in CD4+ effector/memory cells by SGK1 activation. In Life science alliance, 7, . doi:10.26508/lsa.202402890. https://pubmed.ncbi.nlm.nih.gov/39366761/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen