C57BL/6JCya-Igf1rem1flox/Cya
Common Name:
Igf1r-flox
Product ID:
S-CKO-03057
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Igf1r-flox
Strain ID
CKOCMP-16001-Igf1r-B6J-VA
Gene Name
Product ID
S-CKO-03057
Gene Alias
A330103N21Rik; CD221; D930020L01; IGF-1R; hyft
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Igf1rem1flox/Cya mice (Catalog S-CKO-03057) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005671
NCBI RefSeq
NM_010513
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Igf1r, the insulin-like growth factor 1 receptor, is a cell-surface, tyrosine-kinase-containing heterotetramer. It mediates the biological activities of insulin-like growth factors (IGFs), which are crucial for different cellular and organism functions. The IGF1R is linked to numerous cytoplasmic signaling cascades such as the PI3K-AKT pathway, and plays a key role in malignant transformation, cell survival, and growth [1].
In pancreatic β-cells, mouse models with decreased IGF1/IGF1R signaling, like Ames Dwarf mice (lacking growth hormone and having reduced IGF1 levels) and inducible β-cell-specific IGF1R knockdown (βIgf1rKD) mice, showed suppressed senescence, improved glucose clearance, and enhanced insulin-secretory function. Deletion of Igf1R in mouse β-cells also led to the restoration of certain signaling pathways and regulation of genes related to β-cell identity and senescence [2]. In non-small cell lung cancer (NSCLC), circ_PPAPDC1A promotes Osimertinib resistance by sponging miR-30a-3p to activate the Igf1r/PI3K/AKT/mTOR pathway [3].
In conclusion, Igf1r is essential for mediating IGF-related cellular functions. Studies using gene-knockdown mouse models have revealed its role in pancreatic β-cell function and senescence, as well as in NSCLC drug resistance. These findings contribute to understanding diseases related to cellular senescence and cancer drug resistance, potentially providing new therapeutic targets.
References:
1. Werner, Haim, Sarfstein, Rive, Bruchim, Ilan. 2019. Investigational IGF1R inhibitors in early stage clinical trials for cancer therapy. In Expert opinion on investigational drugs, 28, 1101-1112. doi:10.1080/13543784.2019.1694660. https://pubmed.ncbi.nlm.nih.gov/31731883/
2. Iwasaki, Kanako, Lalani, Benjamin, Kahng, Jiho, Kulkarni, Rohit N, Aguayo-Mazzucato, Cristina. 2023. Decreased IGF1R attenuates senescence and improves function in pancreatic β-cells. In Frontiers in endocrinology, 14, 1203534. doi:10.3389/fendo.2023.1203534. https://pubmed.ncbi.nlm.nih.gov/37441495/
3. Tang, Yi-Fang, Liu, Zheng-Hua, Zhang, Lei-Yi, Hu, Chun-Hong, Zou, Fang-Wen. 2024. circ_PPAPDC1A promotes Osimertinib resistance by sponging the miR-30a-3p/ IGF1R pathway in non-small cell lung cancer (NSCLC). In Molecular cancer, 23, 91. doi:10.1186/s12943-024-01998-w. https://pubmed.ncbi.nlm.nih.gov/38715012/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen