C57BL/6NCya-Il15em1flox/Cya
Common Name
Il15-flox
Product ID
S-CKO-03085
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-16168-Il15-B6N-VA
Status
When using this mouse strain in a publication, please cite “Il15-flox Mouse (Catalog S-CKO-03085) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Il15-flox
Strain ID
CKOCMP-16168-Il15-B6N-VA
Gene Name
Product ID
S-CKO-03085
Gene Alias
IL-15
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000209363
NCBI RefSeq
NM_001254747
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Overview of Gene Research
Il15, Interleukin 15, is a cytokine belonging to the immunoregulatory cytokines family. It serves multiple functions, such as dictating T cell response, regulating tissue repair and B cell homing, modulating inflammation, and activating NK cells. IL-15 is unique due to its wide expression, tightly regulated secretion, trans-presentation, and therapeutic potential [3].
In cancer research, transgenic expression of IL15 and IL21 enhanced glypican-3-CAR T cells' antitumor properties against hepatocellular carcinoma, with increased proliferation related to TCF-1 upregulation [1]. IL15 synergizes with radiotherapy to reprogram the tumor immune contexture via a dendritic cell connection [2]. Exercise-induced engagement of the IL-15/IL-15Rα axis promotes anti-tumor immunity in pancreatic cancer [4]. In combination immunotherapy, IL15 with other agents like anti-CTLA-4 and anti-PD-L1 showed better effects in tumor-bearing mice, and clinical trials of such combinations are ongoing [5]. Also, co-expressing IL15 with CXCL10 in CAR-T cells enhances anti-tumor effects in gastric cancer [6]. Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation, improving their therapeutic feasibility [7].
In conclusion, Il15 is crucial for the activation and survival of anti-tumor effectors like CD8+ T and NK cells. Its role in enhancing T-cell expansion, survival, and function makes it a promising target in cancer immunotherapy. Studies on Il15 also contribute to understanding immune-related processes in autoimmunity, although anti-IL-15 therapies have not yet been successful in humans [8].
References：
1. Batra, Sai Arun, Rathi, Purva, Guo, Linjie, Metelitsa, Leonid S, Heczey, Andras. 2020. Glypican-3-Specific CAR T Cells Coexpressing IL15 and IL21 Have Superior Expansion and Antitumor Activity against Hepatocellular Carcinoma. In Cancer immunology research, 8, 309-320. doi:10.1158/2326-6066.CIR-19-0293. https://pubmed.ncbi.nlm.nih.gov/31953246/
2. Pilones, Karsten A, Charpentier, Maud, Garcia-Martinez, Elena, Demaria, Sandra. 2020. IL15 synergizes with radiotherapy to reprogram the tumor immune contexture through a dendritic cell connection. In Oncoimmunology, 9, 1790716. doi:10.1080/2162402X.2020.1790716. https://pubmed.ncbi.nlm.nih.gov/32934886/
3. Patidar, Manoj, Yadav, Naveen, Dalai, Sarat K. 2016. Interleukin 15: A key cytokine for immunotherapy. In Cytokine & growth factor reviews, 31, 49-59. doi:10.1016/j.cytogfr.2016.06.001. https://pubmed.ncbi.nlm.nih.gov/27325459/
4. Kurz, Emma, Hirsch, Carolina Alcantara, Dalton, Tanner, Winograd, Rafael, Bar-Sagi, Dafna. 2022. Exercise-induced engagement of the IL-15/IL-15Rα axis promotes anti-tumor immunity in pancreatic cancer. In Cancer cell, 40, 720-737.e5. doi:10.1016/j.ccell.2022.05.006. https://pubmed.ncbi.nlm.nih.gov/35660135/
5. Waldmann, Thomas A, Dubois, Sigrid, Miljkovic, Milos D, Conlon, Kevin C. 2020. IL-15 in the Combination Immunotherapy of Cancer. In Frontiers in immunology, 11, 868. doi:10.3389/fimmu.2020.00868. https://pubmed.ncbi.nlm.nih.gov/32508818/
6. Nie, Siyue, Song, Yujie, Hu, Kun, Zhou, Zishan, Jiao, Shunchang. 2024. CXCL10 and IL15 co-expressing chimeric antigen receptor T cells enhance anti-tumor effects in gastric cancer by increasing cytotoxic effector cell accumulation and survival. In Oncoimmunology, 13, 2358590. doi:10.1080/2162402X.2024.2358590. https://pubmed.ncbi.nlm.nih.gov/38812569/
7. Berjis, Abdulla, Muthumani, Deeksha, Aguilar, Oscar A, June, Carl H, Sheppard, Neil C. 2024. Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation. In Nature communications, 15, 3937. doi:10.1038/s41467-024-47574-0. https://pubmed.ncbi.nlm.nih.gov/38729924/
8. Allard-Chamard, Hugues, Mishra, Hemant K, Nandi, Madhuparna, Ilangumaran, Subburaj, Ramanathan, Sheela. 2020. Interleukin-15 in autoimmunity. In Cytokine, 136, 155258. doi:10.1016/j.cyto.2020.155258. https://pubmed.ncbi.nlm.nih.gov/32919253/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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