C57BL/6JCya-Il1r1em1flox/Cya
Common Name:
Il1r1-flox
Product ID:
S-CKO-03093
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Il1r1-flox
Strain ID
CKOCMP-16177-Il1r1-B6J-VA
Gene Name
Product ID
S-CKO-03093
Gene Alias
CD121a; CD121b; IL-1R-1; IL-1R-alpha; IL-1R1; IL-1RT-1; IL-1RT1; IL-iR; Il1r-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il1r1em1flox/Cya mice (Catalog S-CKO-03093) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027241
NCBI RefSeq
NM_008362
Target Region
Exon 3~4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Il1r1, encoding interleukin 1 receptor type 1, is a key component in the interleukin 1 (IL-1) signaling pathway. The IL-1 pathway is a central mediator for systemic inflammation, and Il1r1 is crucial for IL-1 signaling, which is involved in numerous biological processes including inflammation, immune response, and metabolism [3].
In colorectal cancer, IL1R1+ cancer-associated fibroblasts drive tumor development and immunosuppression. Fibroblast-specific IL1R1 knockout reduces tumor growth in vivo, accompanied by reduced intratumoral Th17 cell infiltration, suggesting its pro-tumorigenic role [1]. In obesity-related studies, IL1R1-deficient mice are completely resistant to the leptin-sensitizing and anti-obesity effects of celastrol, indicating that IL1R1 is a gatekeeper for celastrol's metabolic actions [2]. Also, a missense variant in IL1R1 in a patient with chronic recurrent multifocal osteomyelitis led to unopposed IL-1 signaling due to disrupted binding of the antagonist ligand. Mice with a homologous mutation showed similar hyperinflammation [3].
In conclusion, Il1r1 plays essential roles in inflammation, metabolism, and tumor-related processes. Studies using gene knockout mouse models, such as fibroblast-specific IL1R1 knockout in colorectal cancer and IL1R1-deficient mice in obesity research, have significantly enhanced our understanding of its functions in disease conditions, highlighting its potential as a therapeutic target in various diseases including cancer, auto-inflammatory diseases, and metabolic disorders.
References:
1. Koncina, E, Nurmik, M, Pozdeev, V I, Dolznig, H, Letellier, E. 2023. IL1R1+ cancer-associated fibroblasts drive tumor development and immunosuppression in colorectal cancer. In Nature communications, 14, 4251. doi:10.1038/s41467-023-39953-w. https://pubmed.ncbi.nlm.nih.gov/37460545/
2. Feng, Xudong, Guan, Dongxian, Auen, Thomas, Copps, Kyle D, Ozcan, Umut. 2019. IL1R1 is required for celastrol's leptin-sensitization and antiobesity effects. In Nature medicine, 25, 575-582. doi:10.1038/s41591-019-0358-x. https://pubmed.ncbi.nlm.nih.gov/30833749/
3. Wang, Yusha, Wang, Jun, Zheng, Wenjie, Yu, Xiaomin, Zhou, Qing. 2023. Identification of an IL-1 receptor mutation driving autoinflammation directs IL-1-targeted drug design. In Immunity, 56, 1485-1501.e7. doi:10.1016/j.immuni.2023.05.014. https://pubmed.ncbi.nlm.nih.gov/37315560/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen