C57BL/6NCya-Lrig1em1flox/Cya
Common Name:
Lrig1-flox
Product ID:
S-CKO-03119
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Lrig1-flox
Strain ID
CKOCMP-16206-Lrig1-B6N-VA
Gene Name
Product ID
S-CKO-03119
Gene Alias
D6Bwg0781e; Img; LIG-1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Lrig1em1flox/Cya mice (Catalog S-CKO-03119) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000101126
NCBI RefSeq
NM_008377
Target Region
Exon 12
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Lrig1, or leucine-rich repeats and immunoglobulin-like domains protein 1, is an important regulator in multiple biological processes. It acts as an endogenous feedback regulator of receptor tyrosine kinases (RTKs), especially antagonizing the expression and activities of ERBB and other RTKs [2,4,5]. It is involved in regulating stem cell quiescence and has tumor-suppressive functions in several cancers [2].
In T-cell-specific Lrig1-deleted mice, superior antitumor responses were observed due to the expansion of tumor-specific cytotoxic T lymphocytes (CTLs) with enhanced effector function and survival, suggesting Lrig1 as an inhibitory immune checkpoint receptor [1]. In adult neural stem cells, genetic disruption of Lrig1 in vivo led to enhanced proliferation, indicating its role in regulating the exit from quiescence [3]. In the context of colon development, an inducible knockout of Lrig1 showed that it restrains proliferation within a critical developmental time window [6]. In adult neural stem cells, Lrig1 knockout models demonstrated that Lrig1 controls proliferation through facilitating TGFβ and BMP signalling pathways [7].
In conclusion, Lrig1 plays essential roles in regulating stem cell quiescence, restraining cell proliferation, and acting as a tumor suppressor in some cancers. Mouse knockout models have been crucial in revealing these functions, especially in the areas of cancer immunotherapy, neural stem cell regulation, and colon development [1,2,3,6,7].
References:
1. Ta, Hieu Minh, Roy, Dia, Zhang, Keman, Chan, Timothy, Wang, Li Lily. 2024. LRIG1 engages ligand VISTA and impairs tumor-specific CD8+ T cell responses. In Science immunology, 9, eadi7418. doi:10.1126/sciimmunol.adi7418. https://pubmed.ncbi.nlm.nih.gov/38758807/
2. Ji, Yibing, Kumar, Rahul, Gokhale, Abhiram, Li, Qiuhui, Tang, Dean G. 2021. LRIG1, a regulator of stem cell quiescence and a pleiotropic feedback tumor suppressor. In Seminars in cancer biology, 82, 120-133. doi:10.1016/j.semcancer.2020.12.016. https://pubmed.ncbi.nlm.nih.gov/33476721/
3. Marqués-Torrejón, María Ángeles, Williams, Charles A C, Southgate, Benjamin, Parrinello, Simona, Pollard, Steven M. 2021. LRIG1 is a gatekeeper to exit from quiescence in adult neural stem cells. In Nature communications, 12, 2594. doi:10.1038/s41467-021-22813-w. https://pubmed.ncbi.nlm.nih.gov/33972529/
4. Neirinckx, Virginie, Hedman, Hakan, Niclou, Simone P. 2017. Harnessing LRIG1-mediated inhibition of receptor tyrosine kinases for cancer therapy. In Biochimica et biophysica acta. Reviews on cancer, 1868, 109-116. doi:10.1016/j.bbcan.2017.02.007. https://pubmed.ncbi.nlm.nih.gov/28259645/
5. Wang, Y, Poulin, E J, Coffey, R J. 2013. LRIG1 is a triple threat: ERBB negative regulator, intestinal stem cell marker and tumour suppressor. In British journal of cancer, 108, 1765-70. doi:10.1038/bjc.2013.138. https://pubmed.ncbi.nlm.nih.gov/23558895/
6. Hopton, Rachel E, Jahahn, Nicholas J, Zemper, Anne E. 2023. Lrig1 drives cryptogenesis and restrains proliferation during colon development. In American journal of physiology. Gastrointestinal and liver physiology, 325, G570-G581. doi:10.1152/ajpgi.00094.2023. https://pubmed.ncbi.nlm.nih.gov/37873577/
7. Ouzikov, Stephanie, Edwards, Kyshona M, Anandampillai, Tanvi, Lee, Jeffrey E, Yuzwa, Scott A. 2024. LRIG1 controls proliferation of adult neural stem cells by facilitating TGFβ and BMP signalling pathways. In Communications biology, 7, 845. doi:10.1038/s42003-024-06524-8. https://pubmed.ncbi.nlm.nih.gov/38987622/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen