C57BL/6JCya-Inhbaem1flox/Cya
Common Name:
Inhba-flox
Product ID:
S-CKO-03125
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Inhba-flox
Strain ID
CKOCMP-16323-Inhba-B6J-VB
Gene Name
Product ID
S-CKO-03125
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Inhbaem1flox/Cya mice (Catalog S-CKO-03125) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000042603
NCBI RefSeq
NM_008380
Target Region
Exon 5
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Inhba, encoding inhibin subunit beta A, is a member of the transforming growth factor-beta (TGF-β) superfamily. It is involved in the synthesis of inhibin A, activin A and activin AB, and participates in multiple biological processes like cell proliferation, apoptosis, hormone synthesis, and is associated with pathways such as SMAD2/3 signaling, Hippo signaling, and immune-related pathways [3,5,6].
In ovarian cancer, INHBA(+) cancer-associated fibroblasts generate an immunosuppressive tumor microenvironment. Intraperitoneal injection of the Activin A neutralizing antibody in mouse models attenuated tumor progression and infiltration of pro-tumorigenic cells, and downregulation of INHBA in human ovarian CAFs inhibited pro-tumorigenic functions [1]. In esophageal squamous cancer cells, knockdown of IGF2BP1, which stabilizes INHBA mRNA, inhibited cell invasion and migration [2]. In gastric cancer, disrupting the GLI1/INHBA positive feedback loop could inhibit tumorigenesis in vivo [4]. In colon cancer, downregulation of INHBA enhanced 5-FU sensitivity, inhibited cell proliferation, and promoted apoptosis, and INHBA conferred 5-FU chemoresistance through negative regulation of Hippo signaling [6].
In conclusion, Inhba plays crucial roles in various biological functions including cell growth, apoptosis, and hormone regulation. Studies using gene-targeted mouse models and other loss-of-function experiments have revealed its significance in multiple cancer types such as ovarian, esophageal, gastric, and colon cancer, highlighting its potential as a therapeutic target in these diseases.
References:
1. Hu, Ye, Recouvreux, Maria Sol, Haro, Marcela, Karlan, Beth Y, Orsulic, Sandra. 2024. INHBA(+) cancer-associated fibroblasts generate an immunosuppressive tumor microenvironment in ovarian cancer. In NPJ precision oncology, 8, 35. doi:10.1038/s41698-024-00523-y. https://pubmed.ncbi.nlm.nih.gov/38360876/
2. Wang, Juan-Juan, Chen, Ding-Xiong, Zhang, Yu, Hao, Jia-Jie, Wang, Ming-Rong. 2023. Elevated expression of the RNA-binding protein IGF2BP1 enhances the mRNA stability of INHBA to promote the invasion and migration of esophageal squamous cancer cells. In Experimental hematology & oncology, 12, 75. doi:10.1186/s40164-023-00429-8. https://pubmed.ncbi.nlm.nih.gov/37644505/
3. Abdel Mouti, Mai, Pauklin, Siim. 2021. TGFB1/INHBA Homodimer/Nodal-SMAD2/3 Signaling Network: A Pivotal Molecular Target in PDAC Treatment. In Molecular therapy : the journal of the American Society of Gene Therapy, 29, 920-936. doi:10.1016/j.ymthe.2021.01.002. https://pubmed.ncbi.nlm.nih.gov/33429081/
4. Sun, Jingguo, Zhu, Wenshuai, Luan, Muhua, Wang, Yunshan, Jia, Yanfei. 2024. Positive GLI1/INHBA feedback loop drives tumor progression in gastric cancer. In Cancer science, 115, 2301-2317. doi:10.1111/cas.16193. https://pubmed.ncbi.nlm.nih.gov/38676428/
5. Bao, Yongjin, Yao, Xiaolei, Li, Xiaodan, Liu, Zifei, Wang, Feng. 2021. INHBA transfection regulates proliferation, apoptosis and hormone synthesis in sheep granulosa cells. In Theriogenology, 175, 111-122. doi:10.1016/j.theriogenology.2021.09.004. https://pubmed.ncbi.nlm.nih.gov/34537472/
6. Zhang, Zhan, Chen, Lili, Yang, Qiao, Wang, Youqun, Huang, Hui. 2024. INHBA regulates Hippo signaling to confer 5-FU chemoresistance mediated by cellular senescence in colon cancer cells. In The international journal of biochemistry & cell biology, 171, 106570. doi:10.1016/j.biocel.2024.106570. https://pubmed.ncbi.nlm.nih.gov/38588888/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen