C57BL/6JCya-Irf4em1flox/Cya
Common Name:
Irf4-flox
Product ID:
S-CKO-03140
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Irf4-flox
Strain ID
CKOCMP-16364-Irf4-B6J-VA
Gene Name
Product ID
S-CKO-03140
Gene Alias
IRF-4; LSIRF; NF-EM5; Spip
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Irf4em1flox/Cya mice (Catalog S-CKO-03140) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021784
NCBI RefSeq
NM_013674
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Irf4, a transcription factor in the interferon regulatory factor (IRF) family, is crucial for immune cell development and function [2][3][4]. It mediates critical immune responses via interactions with upstream signaling pathways like the T-cell receptor and B-cell receptor pathways, and their binding partners in normal lymphocyte development and immunity [2]. It also plays key roles in controlling B cell to plasma cell differentiation and immunoglobulin class-switching [6].
Deleting Arid1a in activated murine B cells, which is required for Irf4 expression, disrupts Irf4-dependent transcriptional networks and blocks plasma cell differentiation [1]. A knock-in mouse model of heterozygous T95R mutation in Irf4 showed a severe defect in antibody production, consistent with the autosomal dominant combined immunodeficiency observed in patients with this mutation [3]. Reducing Irf4 expression in antigen-specific T cells from mice infected with lymphocytic choriomeningitis virus restored their functional and metabolic properties and promoted memory-like T cell development, indicating Irf4 promotes T cell exhaustion during chronic infection [5].
In conclusion, Irf4 is essential for immune cell development, lymphocyte activation, and plasma cell differentiation. Mouse models, such as gene-knockout and knock-in models, have been instrumental in revealing its role in diseases like multiple myeloma, combined immunodeficiency, and during chronic infections, providing insights into its function and potential therapeutic targets.
References:
1. Bolomsky, Arnold, Ceribelli, Michele, Scheich, Sebastian, Muppidi, Jagan, Young, Ryan M. 2024. IRF4 requires ARID1A to establish plasma cell identity in multiple myeloma. In Cancer cell, 42, 1185-1201.e14. doi:10.1016/j.ccell.2024.05.026. https://pubmed.ncbi.nlm.nih.gov/38906156/
2. Wong, Regina Wan Ju, Ong, Jolynn Zu Lin, Theardy, Madelaine Skolastika, Sanda, Takaomi. 2022. IRF4 as an Oncogenic Master Transcription Factor. In Cancers, 14, . doi:10.3390/cancers14174314. https://pubmed.ncbi.nlm.nih.gov/36077849/
3. Fornes, Oriol, Jia, Alicia, Kuehn, Hye Sun, Turvey, Stuart E, Wang, Ji-Yang. 2023. A multimorphic mutation in IRF4 causes human autosomal dominant combined immunodeficiency. In Science immunology, 8, eade7953. doi:10.1126/sciimmunol.ade7953. https://pubmed.ncbi.nlm.nih.gov/36662884/
4. Thouenon, Romane, Kracker, Sven. 2023. Human inborn errors of immunity associated with IRF4. In Frontiers in immunology, 14, 1236889. doi:10.3389/fimmu.2023.1236889. https://pubmed.ncbi.nlm.nih.gov/37809068/
5. Man, Kevin, Gabriel, Sarah S, Liao, Yang, Shi, Wei, Kallies, Axel. 2017. Transcription Factor IRF4 Promotes CD8+ T Cell Exhaustion and Limits the Development of Memory-like T Cells during Chronic Infection. In Immunity, 47, 1129-1141.e5. doi:10.1016/j.immuni.2017.11.021. https://pubmed.ncbi.nlm.nih.gov/29246443/
6. Agnarelli, Alessandro, Chevassut, Tim, Mancini, Erika J. 2018. IRF4 in multiple myeloma-Biology, disease and therapeutic target. In Leukemia research, 72, 52-58. doi:10.1016/j.leukres.2018.07.025. https://pubmed.ncbi.nlm.nih.gov/30098518/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen