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C57BL/6JCya-Itgb5em1flox/Cya
Common Name:
Itgb5-flox
Product ID:
S-CKO-03167
Background:
C57BL/6JCya
Product Type
Age
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Sex
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Basic Information
Strain Name
Itgb5-flox
Strain ID
CKOCMP-16419-Itgb5-B6J-VA
Gene Name
Itgb5
Product ID
S-CKO-03167
Gene Alias
ESTM23; [b]-5; [b]5; [b]5A; [b]5B; beta-5; beta5
Background
C57BL/6JCya
NCBI ID
16419
Modification
Conditional knockout
Chromosome
16
Phenotype
MGI:96614
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itgb5em1flox/Cya mice (Catalog S-CKO-03167) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069345
NCBI RefSeq
NM_010580
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Itgb5, or integrin β5, is a key component of integrin proteins, which play essential roles in cell adhesion, migration, and signaling. Integrins are involved in numerous cellular processes and pathways, such as cell-matrix interactions, focal adhesion, and the regulation of various signaling cascades like the TGF-β, Wnt, and MAPK pathways. Itgb5 is crucial for normal physiological functions and its dysregulation is associated with various diseases [1,4,6].

In cancer research, Itgb5 has been shown to promote metastasis in gastric cancer. Knockdown of Itgb5 impairs gastric cancer cell metastasis both in vitro and in vivo. It acts as a scaffold, interacting with TGFBR2 and SNX17 to facilitate TGFBR2 endosomal recycling, enhancing TGF-β-mediated epithelial-mesenchymal transition and metastasis [1]. In pancreatic adenocarcinoma, knockdown of Itgb5 increases cell sensitivity to radiation by promoting DNA damage repair and the MEK/ERK signaling pathway [2]. In hepatocellular carcinoma, Itgb5 confers sorafenib resistance through a positive feedback loop involving EPS15, EGFR, and CSNK1A1 [4]. In colorectal cancer, silencing Itgb5 inhibits cell proliferation, invasion, and migration by blocking the Wnt/β-catenin signaling pathway and epithelial-mesenchymal transition [6].

In conclusion, Itgb5 is essential for normal cell-matrix interactions and signal transduction. Through gene-knockout or knockdown models in cancer research, it has been revealed that Itgb5 plays a significant role in promoting cancer metastasis, resistance to therapy, and cancer cell growth. Understanding the functions of Itgb5 provides potential therapeutic targets for treating various cancers. Additionally, Itgb5 may be involved in other diseases such as osteoarthritis where it shows diagnostic potential [3], and in the context of Escherichia coli F4ac-induced diarrhea in piglets [5].

References:
1. Lei, Tianxiang, Lin, Yingying, Lai, Xiaofan, Feng, Wei, Song, Wu. 2024. ITGB5 facilitates gastric cancer metastasis by promoting TGFBR2 endosomal recycling. In Cancer letters, 592, 216953. doi:10.1016/j.canlet.2024.216953. https://pubmed.ncbi.nlm.nih.gov/38729557/
2. Wen, Xin, Chen, Si, Chen, Xueting, Ding, Xin, Zhang, Longzhen. 2022. ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway. In Frontiers in oncology, 12, 887068. doi:10.3389/fonc.2022.887068. https://pubmed.ncbi.nlm.nih.gov/36249018/
3. Hu, Shouchao, Huang, Jianxin, Wang, Wenzhi. . ITGB5 as a Potential Diagnostic Biomarker for Osteoarthritis. In Journal of musculoskeletal & neuronal interactions, 25, 86-100. doi:10.22540/JMNI-25-086. https://pubmed.ncbi.nlm.nih.gov/40024232/
4. Gu, Li, Jin, Xin, Liang, Huaiyuan, Yang, Chong, Zhang, Yu. 2023. Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex. In Pharmacological research, 192, 106789. doi:10.1016/j.phrs.2023.106789. https://pubmed.ncbi.nlm.nih.gov/37149115/
5. Wang, Wenwen, Liu, Yang, Tang, Hui, Yu, Ying, Zhang, Qin. 2019. ITGB5 Plays a Key Role in Escherichia coli F4ac-Induced Diarrhea in Piglets. In Frontiers in immunology, 10, 2834. doi:10.3389/fimmu.2019.02834. https://pubmed.ncbi.nlm.nih.gov/31921118/
6. Chen, Zhihua, Fang, Yuan, Zhong, Shuwu, Yang, Xiaoyu, Chen, Shaoqin. 2025. ITGB5 is a prognostic factor in colorectal cancer and promotes cancer progression and metastasis through the Wnt signaling pathway. In Scientific reports, 15, 9225. doi:10.1038/s41598-025-93081-7. https://pubmed.ncbi.nlm.nih.gov/40097546/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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