C57BL/6JCya-Itkem1flox/Cya
Common Name:
Itk-flox
Product ID:
S-CKO-03175
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Itk-flox
Strain ID
CKOCMP-16428-Itk-B6J-VA
Gene Name
Product ID
S-CKO-03175
Gene Alias
Emt; Tcsk; Tsk
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itkem1flox/Cya mice (Catalog S-CKO-03175) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109237
NCBI RefSeq
NM_001281965
Target Region
Exon 2~3
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Itk, also known as IL-2-inducible T-cell kinase, is a non-receptor tyrosine kinase of the Tec family. It plays a crucial role in T-cell receptor signaling pathways, regulating processes such as phospholipase C-gamma1 activation, actin polymerization, and T-cell proliferation and differentiation [3,4,5]. Itk is also involved in the secretion of various pro-inflammatory cytokines like IL-2, IL-4, IL-5, IL-10, and IL-13, and in the development of effector T-cells, thus being of great biological importance in immune responses [4,5]. Genetic models, such as gene knockout (KO) mouse models, have been instrumental in studying Itk.
In Itk-deficient mice, CD4+ T cells showed a bias against Th2 differentiation and an inability to produce Th2 cytokines in a recall response, indicating Itk's role in Th2 cell development [3]. In a collagen-induced arthritis (CIA) model, an Itk inhibitor alleviated the disease, reduced antigen-specific antibody production, and rebalanced Th17 and regulatory T (Treg) cells, suggesting Itk's importance in rheumatoid arthritis development [1]. In T-cell lymphoma studies, the development of a potent Itk degrader, BSJ-05-037, blocked NF-kB/GATA-3 signaling and increased the sensitivity of lymphoma cells to chemotherapy, highlighting Itk's role in T-cell lymphomas [2].
In conclusion, Itk is essential for T-cell signaling, T-cell-mediated immune responses, and the secretion of multiple cytokines. KO mouse models have revealed its significance in diseases such as rheumatoid arthritis and T-cell lymphomas. Understanding Itk's functions through these models provides insights into the pathogenesis of these diseases and potential therapeutic strategies targeting Itk [1,2,3].
References:
1. Chen, Ye, Liang, Rongzhen, Shi, Xiaoyi, Pan, Yunfeng, Zheng, Song Guo. 2023. Targeting kinase ITK treats autoimmune arthritis via orchestrating T cell differentiation and function. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 169, 115886. doi:10.1016/j.biopha.2023.115886. https://pubmed.ncbi.nlm.nih.gov/37992572/
2. Jiang, Baishan, Weinstock, David M, Donovan, Katherine A, Gray, Nathanael S, Wu, Wenchao. 2023. ITK degradation to block T cell receptor signaling and overcome therapeutic resistance in T cell lymphomas. In Cell chemical biology, 30, 383-393.e6. doi:10.1016/j.chembiol.2023.03.007. https://pubmed.ncbi.nlm.nih.gov/37015223/
3. Kosaka, Yoko, Felices, Martin, Berg, Leslie J. 2006. Itk and Th2 responses: action but no reaction. In Trends in immunology, 27, 453-60. doi:. https://pubmed.ncbi.nlm.nih.gov/16931156/
4. Lo, Ho Yin. . Itk inhibitors: a patent review. In Expert opinion on therapeutic patents, 20, 459-69. doi:10.1517/13543771003674409. https://pubmed.ncbi.nlm.nih.gov/20218931/
5. Sahu, Nisebita, August, Avery. . ITK inhibitors in inflammation and immune-mediated disorders. In Current topics in medicinal chemistry, 9, 690-703. doi:. https://pubmed.ncbi.nlm.nih.gov/19689375/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen