C57BL/6JCya-Itpr2em1flox/Cya
Common Name:
Itpr2-flox
Product ID:
S-CKO-03183
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Itpr2-flox
Strain ID
CKOCMP-16439-Itpr2-B6J-VA
Gene Name
Product ID
S-CKO-03183
Gene Alias
InsP3R-2; InsP3R-5; Ip3r2; Itpr5; insP3R2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itpr2em1flox/Cya mice (Catalog S-CKO-03183) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053273
NCBI RefSeq
NM_019923
Target Region
Exon 11
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Itpr2, short for inositol 1,4,5-trisphosphate receptor, type 2, is an endoplasmic reticulum calcium-release channel. It plays a crucial role in calcium fluxes from the ER to the mitochondria, which are involved in multiple biological processes such as cellular senescence, myelination, and calcium homeostasis regulation. These processes are linked to various signaling pathways like the MAPK/ERK-CDK6/cyclin D1 axis [1,2].
Itpr2 knockout (KO) mice have been instrumental in understanding its functions. In Itpr2 KO mice, there is improved aging, with increased lifespan, better metabolic stress response, less immunosenescence, and reduced liver steatosis and fibrosis due to decreased cellular senescence. Ablation of ITPR2 also decreases the number of contacts between the mitochondria and the ER, and forced contacts can induce premature senescence [1]. In OL-specific Itpr2 KO adolescent mice, Ca2+ homeostasis is disturbed, myelination is inhibited, and anxiety/depressive-like behaviors are observed, accompanied by interrupted OPC proliferation [2]. Astrocytic calcium-deficient Itpr2-/-mice display abnormal resting-state functional connectivity in depression-related networks, especially in mPFC-related pathways [3].
In conclusion, Itpr2 is essential for maintaining normal physiological functions related to calcium-mediated processes. Studies using Itpr2 KO mouse models have provided insights into its role in aging, myelin-associated psychiatric disorders, and depression. These findings contribute to understanding the underlying mechanisms of these conditions and may offer potential therapeutic targets.
References:
1. Ziegler, Dorian V, Vindrieux, David, Goehrig, Delphine, Martin, Nadine, Bernard, David. 2021. Calcium channel ITPR2 and mitochondria-ER contacts promote cellular senescence and aging. In Nature communications, 12, 720. doi:10.1038/s41467-021-20993-z. https://pubmed.ncbi.nlm.nih.gov/33526781/
2. Zhang, Ming, Zhi, Na, Feng, Jiaxiang, Wu, Shengxi, Zhao, Xianghui. 2024. ITPR2 Mediated Calcium Homeostasis in Oligodendrocytes is Essential for Myelination and Involved in Depressive-Like Behavior in Adolescent Mice. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2306498. doi:10.1002/advs.202306498. https://pubmed.ncbi.nlm.nih.gov/38476116/
3. Liu, Jiaming, Mo, Jia-Wen, Wang, Xunda, Feng, Yanqiu, Cao, Xiong. 2022. Astrocyte dysfunction drives abnormal resting-state functional connectivity in depression. In Science advances, 8, eabo2098. doi:10.1126/sciadv.abo2098. https://pubmed.ncbi.nlm.nih.gov/36383661/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen