C57BL/6JCya-Junbem1flox/Cya
Common Name:
Junb-flox
Product ID:
S-CKO-03199
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Junb-flox
Strain ID
CKOCMP-16477-Junb-B6J-VA
Gene Name
Product ID
S-CKO-03199
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Junbem1flox/Cya mice (Catalog S-CKO-03199) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064922
NCBI RefSeq
NM_008416
Target Region
Exon 1
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
JunB, a proto-oncogene, is a crucial member of the dimeric activator protein-1 (AP-1) complex. It plays significant roles in various physiological processes such as placental formation, cardiovascular development, myelopoiesis, angiogenesis, endochondral ossification, and epidermis tissue homeostasis. It is also involved in regulating innate and adaptive immune responses by influencing immune cell differentiation and cytokine secretion, and is implicated in tumorigenesis through controlling cell proliferation, differentiation, senescence, and metastasis [1].
In male mice, depletion of JunB in adipocytes increases the fraction of adipocytes with high thermogenic capacity, leading to enhanced energy expenditure and protection against diet-induced obesity and insulin resistance. JunB antagonizes the stimulatory effects of PPARγ coactivator-1α on high-thermogenic adipocyte formation [2].
In macrophage-specific JunB knockdown mice, inhibition of JunB blunts the administration time-dependent effect of ConA and attenuates liver injury, indicating JunB promotes macrophage inflammation through regulating AKT and extracellular signal-regulated kinase (ERK) signalling pathways [3].
In triple-negative breast cancer (TNBC), NAT10 upregulates JunB expression via increasing ac4C modification on its mRNA, and JunB further up-regulates LDHA expression to facilitate glycolysis and create an immunosuppressive tumor microenvironment [4].
In preeclampsia, interference with JUNB expression in macrophages promotes M2 polarization, which benefits trophoblast invasion, migration, and angiogenesis. JUNB regulates the MIIP/PI3K/AKT pathway [5].
In CAR-T cells, downregulation of JunB by MEK inhibition prevents cell exhaustion and terminal differentiation [6].
In T helper cells, Junb-deficient CD4+ T cells show impaired accumulation and increased sensitivity to apoptosis, and JunB directly inhibits apoptosis-related genes [7].
In a syngeneic spontaneous breast cancer metastasis model, stromal JUNB acts as a suppressor of distant metastasis, and its deficiency leads to increased influx of myeloid cells and upregulation of angiogenesis-promoting proteins in neutrophils [8].
In conclusion, JunB has diverse essential biological functions, playing a role in metabolism, immune responses, and tumorigenesis. Studies using gene knockout or conditional knockout mouse models have revealed its significance in specific disease areas such as obesity, liver injury, TNBC, preeclampsia, and breast cancer metastasis, enhancing our understanding of related biological processes and providing potential therapeutic targets.
References:
1. Ren, Fu-Jia, Cai, Xiao-Yu, Yao, Yao, Fang, Guo-Ying. 2023. JunB: a paradigm for Jun family in immune response and cancer. In Frontiers in cellular and infection microbiology, 13, 1222265. doi:10.3389/fcimb.2023.1222265. https://pubmed.ncbi.nlm.nih.gov/37731821/
2. Zhang, Xing, Ding, Xiaofeng, Wang, Chunqing, Wang, Qiong A, Liu, Meilian. 2024. Depletion of JunB increases adipocyte thermogenic capacity and ameliorates diet-induced insulin resistance. In Nature metabolism, 6, 78-93. doi:10.1038/s42255-023-00945-1. https://pubmed.ncbi.nlm.nih.gov/38191667/
3. Liu, Zhaiyi, Zhang, Jiayang, Li, Shuyao, Yang, Guangrui, Chen, Lihong. 2023. Circadian control of ConA-induced acute liver injury and inflammatory response via Bmal1 regulation of Junb. In JHEP reports : innovation in hepatology, 5, 100856. doi:10.1016/j.jhepr.2023.100856. https://pubmed.ncbi.nlm.nih.gov/37791375/
4. Li, Guozheng, Ma, Xin, Sui, Shiyao, Tao, Weiyang, Xu, Shouping. 2024. NAT10/ac4C/JunB facilitates TNBC malignant progression and immunosuppression by driving glycolysis addiction. In Journal of experimental & clinical cancer research : CR, 43, 278. doi:10.1186/s13046-024-03200-x. https://pubmed.ncbi.nlm.nih.gov/39363363/
5. Jiang, Peiyue, Zhu, Xiaojun, Jiang, Ying, Li, Hetong, Luo, Qiong. 2024. Targeting JUNB to modulate M2 macrophage polarization in preeclampsia. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167194. doi:10.1016/j.bbadis.2024.167194. https://pubmed.ncbi.nlm.nih.gov/38663490/
6. Wang, Xiujian, Tao, Xiao, Chen, Pengjie, Qian, Pengxu, Huang, He. 2024. MEK inhibition prevents CAR-T cell exhaustion and differentiation via downregulation of c-Fos and JunB. In Signal transduction and targeted therapy, 9, 293. doi:10.1038/s41392-024-01986-y. https://pubmed.ncbi.nlm.nih.gov/39438476/
7. Hsieh, Tsunghan, Sasaki, Daiki, Taira, Naoyuki, Miyagi, Mio, Ishikawa, Hiroki. 2022. JunB Is Critical for Survival of T Helper Cells. In Frontiers in immunology, 13, 901030. doi:10.3389/fimmu.2022.901030. https://pubmed.ncbi.nlm.nih.gov/35837408/
8. Wutschka, Juliane, Kast, Bettina, Sator-Schmitt, Melanie, Angel, Peter, Schorpp-Kistner, Marina. 2021. JUNB suppresses distant metastasis by influencing the initial metastatic stage. In Clinical & experimental metastasis, 38, 411-423. doi:10.1007/s10585-021-10108-9. https://pubmed.ncbi.nlm.nih.gov/34282521/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen