C57BL/6JCya-Khkem1flox/Cya
Common Name:
Khk-flox
Product ID:
S-CKO-03249
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Khk-flox
Strain ID
CKOCMP-16548-Khk-B6J-VA
Gene Name
Product ID
S-CKO-03249
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Khkem1flox/Cya mice (Catalog S-CKO-03249) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031053
NCBI RefSeq
NM_001310524
Target Region
Exon 3~5
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Khk, also known as ketohexokinase, is the primary enzyme for fructose metabolism. It catalyzes the first step of fructolysis, phosphorylating fructose to fructose-1-phosphate. This process is crucial in various metabolic pathways, including de novo lipogenesis (DNL), fatty acid oxidation, and glycolysis, and is of great biological importance in the context of metabolic diseases and cancer [1,2,3,4,5,6]. Genetic models, such as KO/CKO mouse models, have been valuable in studying Khk's function.
In animal models, inhibition of Khk using a small molecule inhibitor (PF-06835919) reversed fructose-induced metabolic dysfunction, including insulin resistance, hypertriglyceridemia, and hepatic steatosis. This coincided with reduced DNL and inactivation of the lipogenic transcription factor ChREBP [2]. In mice, a liver-specific knockdown of Khk-C improved the NAFLD activity score and had a profound effect on the hepatic transcriptome when consuming fructose with a high-fat diet [4]. Also, pharmacological inhibition of ketohexokinase decreased circulating lysophosphatidylcholines (LPCs) and prevented fructose-mediated tumour growth in vivo, as cancer cells utilized hepatocyte-derived LPCs for growth [7].
In conclusion, Khk plays a key role in fructose metabolism and is involved in multiple biological processes related to metabolic diseases and cancer. Studies using Khk KO/CKO mouse models have revealed its significance in conditions such as metabolic syndrome-related liver diseases and tumour growth, providing insights into potential therapeutic strategies targeting Khk in these disease areas.
References:
1. Herman, Mark A, Birnbaum, Morris J. 2021. Molecular aspects of fructose metabolism and metabolic disease. In Cell metabolism, 33, 2329-2354. doi:10.1016/j.cmet.2021.09.010. https://pubmed.ncbi.nlm.nih.gov/34619074/
2. Gutierrez, Jemy A, Liu, Wei, Perez, Sylvie, Birnbaum, Morris J, Tesz, Gregory J. 2021. Pharmacologic inhibition of ketohexokinase prevents fructose-induced metabolic dysfunction. In Molecular metabolism, 48, 101196. doi:10.1016/j.molmet.2021.101196. https://pubmed.ncbi.nlm.nih.gov/33667726/
3. Helsley, Robert N, Park, Se-Hyung, Vekaria, Hemendra J, Kahn, C Ronald, Softic, Samir. 2023. Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation. In Journal of hepatology, 79, 25-42. doi:10.1016/j.jhep.2023.02.010. https://pubmed.ncbi.nlm.nih.gov/36822479/
4. Park, Se-Hyung, Helsley, Robert N, Fadhul, Taghreed, Kahn, C Ronald, Softic, Samir. 2023. Fructose induced KHK-C can increase ER stress independent of its effect on lipogenesis to drive liver disease in diet-induced and genetic models of NAFLD. In Metabolism: clinical and experimental, 145, 155591. doi:10.1016/j.metabol.2023.155591. https://pubmed.ncbi.nlm.nih.gov/37230214/
5. Softic, Samir, Stanhope, Kimber L, Boucher, Jeremie, Johnson, Richard J, Kahn, C Ronald. 2020. Fructose and hepatic insulin resistance. In Critical reviews in clinical laboratory sciences, 57, 308-322. doi:10.1080/10408363.2019.1711360. https://pubmed.ncbi.nlm.nih.gov/31935149/
6. Krause, Nils, Wegner, Andre. 2020. Fructose Metabolism in Cancer. In Cells, 9, . doi:10.3390/cells9122635. https://pubmed.ncbi.nlm.nih.gov/33302403/
7. Fowle-Grider, Ronald, Rowles, Joe L, Shen, Isabel, Zhang, Jin, Patti, Gary J. 2024. Dietary fructose enhances tumour growth indirectly via interorgan lipid transfer. In Nature, 636, 737-744. doi:10.1038/s41586-024-08258-3. https://pubmed.ncbi.nlm.nih.gov/39633044/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen