C57BL/6JCya-Khsrpem1flox/Cya
Common Name:
Khsrp-flox
Product ID:
S-CKO-03250
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Khsrp-flox
Strain ID
CKOCMP-16549-Khsrp-B6J-VA
Gene Name
Product ID
S-CKO-03250
Gene Alias
6330409F21Rik; Fbp2; Fubp2; Ksrp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Khsrpem1flox/Cya mice (Catalog S-CKO-03250) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007814
NCBI RefSeq
NM_010613
Target Region
Exon 2~4
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
KHSRP, the KH-type splicing regulatory protein, is a multifunctional RNA-binding protein. It is involved in various biological processes such as mRNA decay, alternative splicing, and miRNA biogenesis. It also participates in key pathways like the FAK signaling pathway [1]. KHSRP's functions are of great biological importance, influencing processes from cell differentiation to tumor progression, and genetic models are crucial for studying its effects.
In pancreatic ductal adenocarcinoma (PDAC), KHSRP acts as an m6A-binding protein. High levels of KHSRP predict poor patient survival, and KHSRP deficiency suppresses PDAC growth and metastasis in vivo. It stabilizes FAK pathway mRNAs in an m6A-dependent manner, activating downstream FAK signaling to promote PDAC progression [1].
In mice lacking KHSRP (Khsrp-/ -), there are upregulated mRNAs involved in neuronal morphology, axon guidance, etc. These mice show increased axon growth, dendritic spine density, and synaptic transmission, along with memory consolidation deficits, indicating KHSRP's role in neuronal development [2].
In acute liver failure murine models, knockdown of KHSRP leads to splicing defects and exacerbation of liver injury, while KHSRP protects against ALF by regulating pre-mRNA splicing in vivo [3].
In conclusion, KHSRP plays essential roles in multiple biological processes. Gene knockout mouse models, like Khsrp-/-mice, have revealed its significance in diseases such as PDAC, liver failure, and in neuronal development. Understanding KHSRP's functions provides potential therapeutic targets for relevant diseases.
References:
1. Xu, Zilan, Zhou, Yifan, Liu, Shaoqiu, Zheng, Jian, Zhang, Jialiang. . KHSRP Stabilizes m6A-Modified Transcripts to Activate FAK Signaling and Promote Pancreatic Ductal Adenocarcinoma Progression. In Cancer research, 84, 3602-3616. doi:10.1158/0008-5472.CAN-24-0927. https://pubmed.ncbi.nlm.nih.gov/39120596/
2. Olguin, Sarah L, Patel, Priyanka, Buchanan, Courtney N, Twiss, Jeffery L, Perrone-Bizzozero, Nora I. 2022. KHSRP loss increases neuronal growth and synaptic transmission and alters memory consolidation through RNA stabilization. In Communications biology, 5, 672. doi:10.1038/s42003-022-03594-4. https://pubmed.ncbi.nlm.nih.gov/35798971/
3. Li, Mingxuan, Fang, Qian, Xiao, Pingping, Xiao, Ruijing, Zhou, Rui. 2024. KHSRP ameliorates acute liver failure by regulating pre-mRNA splicing through its interaction with SF3B1. In Cell death & disease, 15, 618. doi:10.1038/s41419-024-06886-1. https://pubmed.ncbi.nlm.nih.gov/39187547/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen