C57BL/6JCya-Kif16bem1flox/Cya
Common Name:
Kif16b-flox
Product ID:
S-CKO-03255
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kif16b-flox
Strain ID
CKOCMP-16558-Kif16b-B6J-VA
Gene Name
Product ID
S-CKO-03255
Gene Alias
8430434E15Rik; mKIAA1590
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kif16bem1flox/Cya mice (Catalog S-CKO-03255) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000230763
NCBI RefSeq
NM_001355123
Target Region
Exon 2~3
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Kif16b, a member of the kinesin-3 family, has a characteristic PhoX homology (PX) domain. It binds to membranes containing phosphatidylinositol-3-phosphate (PI(3)P) and moves along microtubule filaments to the plus end, regulated by coiled coils in the stalk region. Its physiological function involves transporting intracellular cargo, forming endosomal tubules, and is crucial for membrane transport-related pathways [1].
Kif16b knockout (KO) mouse embryos failed to develop epiblast and primitive endoderm lineages and died at the peri-implantation stage, similar to FGFR2 knockout embryos. This indicates that the KIF16B/Rab14 complex is critical for the biosynthetic Golgi-to-endosome traffic of the fibroblast growth factor receptor (FGFR) during early embryonic development [2]. In neurons, loss of KIF16B induced the aggregation of early endosomes and perturbed the trafficking and functioning of receptors like the AMPA and NGF receptors, suggesting its importance in the correct localization of early endosomes in mouse hippocampal neurons [3].
In conclusion, Kif16b is essential for intracellular cargo transport, endosomal tubule formation, and early embryonic development as well as neuronal receptor trafficking. The study of Kif16b KO mouse models has provided insights into its role in early embryonic development and neuronal function, highlighting its significance in understanding the mechanisms of these biological processes and potentially related diseases.
References:
1. Li, Bo-Jie, Chen, Hao, Jiang, Su-Su, Zhang, Cai-Ping, Liao, Duan-Fang. 2020. PX Domain-Containing Kinesin KIF16B and Microtubule-Dependent Intracellular Movements. In The Journal of membrane biology, 253, 101-108. doi:10.1007/s00232-020-00110-9. https://pubmed.ncbi.nlm.nih.gov/32140737/
2. Ueno, Hitoshi, Huang, Xiao, Tanaka, Yosuke, Hirokawa, Nobutaka. . KIF16B/Rab14 molecular motor complex is critical for early embryonic development by transporting FGF receptor. In Developmental cell, 20, 60-71. doi:10.1016/j.devcel.2010.11.008. https://pubmed.ncbi.nlm.nih.gov/21238925/
3. Farkhondeh, Atena, Niwa, Shinsuke, Takei, Yosuke, Hirokawa, Nobutaka. . Characterizing KIF16B in neurons reveals a novel intramolecular "stalk inhibition" mechanism that regulates its capacity to potentiate the selective somatodendritic localization of early endosomes. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 35, 5067-86. doi:10.1523/JNEUROSCI.4240-14.2015. https://pubmed.ncbi.nlm.nih.gov/25810535/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen