C57BL/6JCya-Kif3aem1flox/Cya
Common Name:
Kif3a-flox
Product ID:
S-CKO-03263
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kif3a-flox
Strain ID
CKOCMP-16568-Kif3a-B6J-VA
Gene Name
Product ID
S-CKO-03263
Gene Alias
Kif3; Kifl; Kns3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kif3aem1flox/Cya mice (Catalog S-CKO-03263) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000120613
NCBI RefSeq
NM_008443
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Kif3a, a member of the kinesin-2 family, is a microtubule-oriented motor protein. It is crucial for regulating intracellular transport and microtubule movement, and is associated with pathways like the Wnt/β-catenin pathway [4]. It plays an overall significant role in various biological processes such as cell cycle regulation, organelle distribution, and ciliogenesis [1,5,6]. Genetic models, especially KO/CKO mouse models, have been valuable in studying its functions.
In mouse oocyte meiosis, conditional knockout of Kif3a leads to maturation defects, spindle assembly abnormalities, and organelle distribution disorders. It activates the spindle assembly checkpoint, causing metaphase I arrest. Kif3a depletion also results in aberrant spindle pole organization and reduced microtubule stability [1]. In nasopharyngeal carcinoma (NPC) stem cells, knocking down the Kif3a gene reduces cell vitality and inhibits the malignant phenotype by suppressing HIF-1 and NF-κB expression [2]. Overexpression of Kif3a in NPC cells inhibits proliferation, migration, and invasion by interacting with β-catenin to suppress its nuclear accumulation [3]. In Eriocheir sinensis spermatogenesis, knocking down es-kif3a decreases the expression of es-β-catenin and es-dvl, and changes the location of Es-β-catenin from the nucleus to the cytoplasm [4].
In conclusion, Kif3a is essential for multiple biological functions including meiotic progression, cell cycle regulation, and organelle distribution. Mouse KO/CKO models have revealed its significant roles in oocyte meiosis and in cancer-related processes such as in NPC, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Liu, Jing-Cai, Pan, Zhen-Nan, Ju, Jia-Qian, Wu, Xin, Sun, Shao-Chen. 2024. Kinesin KIF3A regulates meiotic progression and spindle assembly in oocyte meiosis. In Cellular and molecular life sciences : CMLS, 81, 168. doi:10.1007/s00018-024-05213-3. https://pubmed.ncbi.nlm.nih.gov/38587639/
2. Liu, Rongrong, Li, Te, Zhang, Weitao. 2023. Inhibitory mechanism of KIF3A gene on nasopharyngeal carcinoma stem cells. In Cellular and molecular biology (Noisy-le-Grand, France), 69, 84-88. doi:10.14715/cmb/2023.69.8.13. https://pubmed.ncbi.nlm.nih.gov/37715417/
3. Hu, Zhe, Meng, Jinlan, Cai, Hongbing, Li, Xiaojuan, Xu, Yan. 2022. KIF3A inhibits nasopharyngeal carcinoma proliferation, migration and invasion by interacting with β-catenin to suppress its nuclear accumulation. In American journal of cancer research, 12, 5226-5240. doi:. https://pubmed.ncbi.nlm.nih.gov/36504907/
4. Liang, Ya-Jing, Ni, Jia-Hao, Wang, Lan-Min, Tan, Fu-Qing, Yang, Wan-Xi. 2020. KIF3A regulates the Wnt/β-catenin pathway via transporting β-catenin during spermatogenesis in Eriocheir sinensis. In Cell and tissue research, 381, 527-541. doi:10.1007/s00441-020-03220-x. https://pubmed.ncbi.nlm.nih.gov/32458081/
5. Fasawe, Ayoola S, Adams, Jessica M, Engelke, Martin F. 2024. KIF3A tail domain phosphorylation is not required for ciliogenesis in mouse embryonic fibroblasts. In iScience, 27, 109149. doi:10.1016/j.isci.2024.109149. https://pubmed.ncbi.nlm.nih.gov/38405607/
6. Kodani, Andrew, Salomé Sirerol-Piquer, Maria, Seol, Allen, Garcia-Verdugo, Jose Manuel, Reiter, Jeremy F. 2013. Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages. In The EMBO journal, 32, 597-607. doi:10.1038/emboj.2013.3. https://pubmed.ncbi.nlm.nih.gov/23386061/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen