C57BL/6JCya-Klf4em1flox/Cya
Common Name
Klf4-flox
Product ID
S-CKO-03285
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-16600-Klf4-B6J-VA
Status
When using this mouse strain in a publication, please cite “Klf4-flox Mouse (Catalog S-CKO-03285) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Klf4-flox
Strain ID
CKOCMP-16600-Klf4-B6J-VA
Gene Name
Product ID
S-CKO-03285
Gene Alias
EZF, Zie, Gklf
Background
C57BL/6JCya
Gene Full Name
Kruppel-like factor 4 (gut)
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 4 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000107619
NCBI Transcript ID
NM_010637
Target Region
Exon 3~4
Size of Effective Region
~2.3 kb
Overview of Gene Research
Klf4, also known as Krüppel-like factor 4, is a key transcription factor. It contains a zinc finger domain and is predominantly expressed in terminally differentiated epithelial tissues. Klf4 is involved in various biological processes such as cell proliferation, migration, invasion, and differentiation, and participates in multiple signaling pathways. It can act as both a tumor suppressor and an oncogene, depending on the context, and also plays a crucial regulatory role in inflammation, making it an important target for research in cancer and inflammatory diseases [2,3,4].
In lung cancer research, loss of USP10 in mice downregulates Klf4 expression and accelerates KrasG12D-driven lung adenocarcinoma initiation and progression, indicating that USP10-Klf4-TIMP3 axis is a potential therapeutic target in lung cancer [1]. In atherosclerotic lesions, vascular smooth muscle cells (VSMCs) undergo a phenotypic switching in a Klf4-dependent manner. Klf4 enhances the metabolic switch to glycolysis through increasing PFKFB3 expression, and inhibiting glycolysis suppresses Klf4-induced VSMCs phenotypic switching [5].
In conclusion, Klf4 has diverse biological functions, being involved in processes like tumorigenesis, inflammation, and cell phenotypic switching. Gene-knockout mouse models, such as the loss of USP10-mediated down-regulation of Klf4 in lung cancer and Klf4-dependent VSMCs phenotypic switching in atherosclerosis, have significantly contributed to understanding Klf4's role in these disease areas, providing potential therapeutic targets for cancer and atherosclerotic diseases.
References：
1. Wang, Xingyun, Xia, Shilin, Li, Hongchang, Zhang, Lingqiang, Han, Chuanchun. 2019. The deubiquitinase USP10 regulates KLF4 stability and suppresses lung tumorigenesis. In Cell death and differentiation, 27, 1747-1764. doi:10.1038/s41418-019-0458-7. https://pubmed.ncbi.nlm.nih.gov/31748695/
2. Luo, Xiaoya, Zhang, Yue, Meng, Ying, Ji, Ming, Wang, Yongjun. 2022. Prognostic significance of KLF4 in solid tumours: an updated meta-analysis. In BMC cancer, 22, 181. doi:10.1186/s12885-022-09198-9. https://pubmed.ncbi.nlm.nih.gov/35177016/
3. Liang, Yidan, Zhao, Jiamin, Dai, Tengkun, Zhao, Juanjuan, Xu, Lin. 2024. A review of KLF4 and inflammatory disease: Current status and future perspective. In Pharmacological research, 207, 107345. doi:10.1016/j.phrs.2024.107345. https://pubmed.ncbi.nlm.nih.gov/39134187/
4. Ju, Yunjie, Xiao, Wen, Mathis, Bryan James, Shi, Ying. 2025. KLF4: a multifunctional nexus connecting tumor progression and immune regulation. In Frontiers in immunology, 16, 1514780. doi:10.3389/fimmu.2025.1514780. https://pubmed.ncbi.nlm.nih.gov/39995670/
5. Zhang, Xinhua, Zheng, Bin, Zhao, Lingdan, Liu, Huirong, Wen, Jinkun. 2022. KLF4-PFKFB3-driven glycolysis is essential for phenotypic switching of vascular smooth muscle cells. In Communications biology, 5, 1332. doi:10.1038/s42003-022-04302-y. https://pubmed.ncbi.nlm.nih.gov/36470917/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
