C57BL/6JCya-Krasem1flox/Cya
Common Name:
Kras-flox
Product ID:
S-CKO-03297
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kras-flox
Strain ID
CKOCMP-16653-Kras-B6J-VA
Gene Name
Product ID
S-CKO-03297
Gene Alias
K-Ras; K-Ras 2; K-ras; Ki-ras; Kras-2; Kras2; c-K-ras; c-Ki-ras; p21B; ras
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Krasem1flox/Cya mice (Catalog S-CKO-03297) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032399
NCBI RefSeq
NM_021284
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
KRAS, an abbreviation of Kirsten Rat Sarcoma, is a crucial oncogene involved in cellular proliferation and survival. It belongs to the RAS family, existing in three isoforms (K-RAS, H-RAS, and N-RAS), with KRAS being the most commonly mutated. The Ras protein has GTPase activity, cycling between an inactive (GDP-bound) and active (GTP-bound) form. When activated, it triggers intracellular signaling pathways like the mitogen-activated protein kinase (MAPK) pathway, which is vital for cell proliferation and angiogenesis [5].
KRAS mutations occur in about a quarter of all human cancers [2]. In pancreatic cancer, over 90% of pancreatic ductal adenocarcinomas (PDACs) carry KRAS mutations, mainly G12D, G12V, and G12R, which are key in tumor initiation and progression [3,4]. In colorectal cancer, around half of the cases have KRAS mutations, associated with a worse prognosis [6]. Although KRAS has long been considered undruggable, recent developments include covalent inhibitors targeting KRASG12C, which show promise in early clinical trials for KRASG12C mutant pancreatic cancer [1]. However, most KRAS oncoproteins remain challenging to target, and efforts are ongoing to develop inhibitors for other mutations like the KRAS G12D-directed MRTX1133 inhibitor now in clinical trials [3].
In conclusion, KRAS is a master oncogene with a significant role in the development of various cancers, especially pancreatic and colorectal cancers. The study of KRAS-related mouse models, like gene knockout (KO) or conditional knockout (CKO) mouse models, could potentially help in understanding its function and developing more effective therapeutic strategies against KRAS-mutant cancers.
References:
1. Luo, Ji. 2021. KRAS mutation in pancreatic cancer. In Seminars in oncology, 48, 10-18. doi:10.1053/j.seminoncol.2021.02.003. https://pubmed.ncbi.nlm.nih.gov/33676749/
2. Drosten, Matthias, Barbacid, Mariano. . Targeting the MAPK Pathway in KRAS-Driven Tumors. In Cancer cell, 37, 543-550. doi:10.1016/j.ccell.2020.03.013. https://pubmed.ncbi.nlm.nih.gov/32289276/
3. Stickler, Sandra, Rath, Barbara, Hamilton, Gerhard. 2024. Targeting KRAS in pancreatic cancer. In Oncology research, 32, 799-805. doi:10.32604/or.2024.045356. https://pubmed.ncbi.nlm.nih.gov/38686056/
4. Bannoura, Sahar F, Uddin, Md Hafiz, Nagasaka, Misako, El-Rayes, Bassel, Azmi, Asfar S. 2021. Targeting KRAS in pancreatic cancer: new drugs on the horizon. In Cancer metastasis reviews, 40, 819-835. doi:10.1007/s10555-021-09990-2. https://pubmed.ncbi.nlm.nih.gov/34499267/
5. Uprety, Dipesh, Adjei, Alex A. 2020. KRAS: From undruggable to a druggable Cancer Target. In Cancer treatment reviews, 89, 102070. doi:10.1016/j.ctrv.2020.102070. https://pubmed.ncbi.nlm.nih.gov/32711246/
6. Nusrat, Maliha, Yaeger, Rona. 2023. KRAS inhibition in metastatic colorectal cancer: An update. In Current opinion in pharmacology, 68, 102343. doi:10.1016/j.coph.2022.102343. https://pubmed.ncbi.nlm.nih.gov/36638742/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen